product summary
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company name :
MyBioSource
product type :
protein
product name :
MGMT, 1-207aa, Human, His-tag, Recombinant, E Coli
catalog :
MBS203260
quantity :
0.1 mg
price :
375 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203260
products type :
Recombinant Protein
products full name :
MGMT, 1-207aa, Human, His-tag, Recombinant, E Coli
products short name :
MGMT
products name syn :
O-6-methylguanine-DNA methyltransferase; MGMT; EC 2.1.1.63; O-6-methylguanine-DNA-alkyltransferase; O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; MGC107020; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase.
other names :
methylated-DNA--protein-cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
products gene name :
MGMT
other gene names :
MGMT; MGMT; MGMT
uniprot entry name :
MGMT_HUMAN
sequence length :
238
sequence :
MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)
products categories :
Enzymes & Proteases
products description :
O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques.
products references :
Matsukura S., et al. (2001) Annals of Surgical Oncology, 8(10):807--816. Esteller M.,et al. (2002) J Natl Cancer Inst. 2, 26-32.
ncbi gi num :
197304670
ncbi acc num :
NP_002403
ncbi gb acc num :
NM_002412.3
ncbi mol weight :
23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
ncbi pathways :
DNA Damage Reversal Pathway (105857); DNA Repair Pathway (105837)
uniprot summary :
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family. Protein type: Methyltransferase; Apoptosis; DNA repair, damage; Nuclear receptor co-regulator; EC 2.1.1.63. Chromosomal Location of Human Ortholog: 10q26. Cellular Component: nucleoplasm; membrane; nucleus. Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity. Biological Process: response to drug; DNA dealkylation; positive regulation of DNA repair; response to ethanol; response to folic acid; response to toxin; DNA methylation; DNA repair; regulation of caspase activity; DNA ligation
size1 :
0.1 mg
price1 :
375 USD
size2 :
0.5 mg
price2 :
925
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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