Cyclophilin F (PPIF), 30-207aa, Human,Recombinant, E Coli | MyBioSource MBS203258 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Cyclophilin F (PPIF), 30-207aa, Human,Recombinant, E Coli

catalog :

MBS203258

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203258

products type :

Recombinant Protein

products full name :

Cyclophilin F (PPIF), 30-207aa, Human,Recombinant, E Coli

products short name :

Cyclophilin F

products name syn :

Peptidylprolyl isomerase F; CYP3; Cyp-D; PPIF; Peptidylprolyl isomerase F Cyclophilin 3; Cyclophilin D; Cyclophilin F; Cyp D; CYP3; CypD; hCyP3; Peptidylprolyl isomerase F (cyclophilin F); Peptidyl prolyl cis trans isomerase; mitochondral; Peptidylprolyl isomerase F; PPIase; Rotamase.

other names :

peptidyl-prolyl cis-trans isomerase F, mitochondrial; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; cyclophilin 3; cyclophilin D; cyclophilin F; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase, mitochondrial; rotamase F; peptidylprolyl isomerase F; Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F

other gene names :

PPIF; PPIF; CYP3; CypD; CyP-M; Cyp-D; CYP3; PPIase F; CyP-D; CypD; CyP-M

uniprot entry name :

PPIF_HUMAN

sequence length :

207

sequence :

MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS

purity :

> 95% by SDS PAGE

form :

Liquid in 20mM Tris pH 7.5, 1mM DTT, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is > 250 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant Cyclophilin F(PPIF) protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

products categories :

Enzymes & Proteases

products description :

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Bergsma DJ., et al. (1991) J Biol Chem. 266(34):23204-14. Bowles KR., et al. (2000) Genomics 67(2):109-27.

ncbi gi num :

5031987

ncbi acc num :

NP_005720

ncbi gb acc num :

NM_005729.3

ncbi mol weight :

21 kDa (198aa), confirmed by MALDI-TOF.

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Parkinson's Disease Pathway (83098); Toxoplasmosis Pathway (169642); Toxoplasmosis Pathway (169493); CGMP-PKG Signaling Pathway (983748); CGMP-PKG Signaling Pathway (985584)

ncbi summary :

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]

uniprot summary :

PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. Protein type: EC 5.2.1.8; Isomerase; Mitochondrial. Chromosomal Location of Human Ortholog: 10q22.3. Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex. Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; cyclosporin A binding. Biological Process: apoptotic mitochondrial changes; protein peptidyl-prolyl isomerization; protein folding; regulation of mitochondrial membrane permeability; negative regulation of ATPase activity; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage