protein

AK2, 1-239aa, Human,His-tag, Recombinant, E Coli

MBS203256

0.1 mg

310 USD

Recombinant Protein

AK2, 1-239aa, Human,His-tag, Recombinant, E Coli

AK2

ADK2; AK2; Adenylate kinase 2; Adenylate kinase isoenzyme 2; ATP AMP transphosphorylase; EC 2.7.4.3

adenylate kinase 2, mitochondrial isoform a; Adenylate kinase 2, mitochondrial; adenylate kinase 2, mitochondrial; AK 2; AK2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; adenylate kinase isoenzyme 2, mitochondrial; adenylate monophosphate kinase; adenylate kinase 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase;

AK2

AK2; AK2; ADK2; AK 2;

KAD2_HUMAN

239

MGSSHHHHHH SSGLVPRGSH MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI

> 95% by SDS - PAGE

Liquid in 20mM Tris pH 7.5, 5mM DTT, 20% glycerol Molecular Weight: 28.6 kDa (259 aa)

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: > 1.5 units/ml. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 25 degree C. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Prepare a 1.4 ml assay buffer (Assay buffer: 58 mM glycylglycine, 2.0 mM adenosine 5'-diphosphate, 2.3 mM beta-nicotinamide adenine dinucleotide phosphate, 10 mM magnesium chloride, 10 mM glucose, 2 unit hexokinase, 1 unit glucose-6-phosphate dehydrogenase, 0.003% (w/v) BSA, pH 7.5.). - G-6-PDH/Hex (sigma,Cat.No. H-8629). Add 50ul of recombinant Adenylate kinase isoenzyme 2 protein with various concentrations (0.5ug, 1ug) and read the increase in A340nm for 5 minutes.

Enzymes & Proteases

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kohler C., et al. (1999).FEBS Lett. 447(1):10-2. Lee Y., et al. (1998).J Biol Chem. 123(1):47-54.

4502013

NP_001616.1

NM_001625.3

28.6 kDa (259 aa), confirmed by MALDI-TOF.

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway 618580!!Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway 468242!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Nucleotides Pathway 106263!!Purine Metabolism Pathway 82944!!Purine Metabolism Pathway 307!!Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway 161057!!Adenosine Ribonucleotides De Novo Biosynthesis Pathway 835389!!Purine Nucleotides De Novo Biosynthesis Pathway 142277

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]

0.1 mg

310 USD