This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
protein
product name :
Carbonic Anhydrase, 1-220aa, E Coli, His-tag, Recombinant, E Coli
catalog :
MBS203253
quantity :
0.1 mg
price :
310 USD
product information
catalog number :
MBS203253
products type :
Recombinant Protein
products full name :
Carbonic Anhydrase, 1-220aa, E Coli, His-tag, Recombinant, E Coli
products short name :
Carbonic Anhydrase
products name syn :
Carbonate dehydratase; CAN; ECK0125; JW0122; yadF; CA 1; CA I; CA1; CAI; Car 1; Car1; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase B.
other names :
carbonic anhydrase; Carbonic anhydrase 2; carbonic anhydrase; Carbonate dehydratase 2
other gene names :
can; can; ECK0125; JW0122; yadF; cynT2; yadF
uniprot entry name :
CAN_ECOLI
sequence length :
220
sequence :
MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
purity :
> 95% by SDS - PAGE
form :
Liquid in 20mM Tris pH8.0, 1mM DTT, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
products references :
Lindskog S., et al :(1997) Pharmacol Ther; 74(1):1-20. Sawaya MR., et al (2006) J Biol Chem. 281(11):7546-55.
ncbi gi num :
16128119
ncbi acc num :
NP_414668
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P61517
ncbi mol weight :
27kDa (240aa), confirmed by MALDI-TOF.
ncbi pathways :
Nitrogen Metabolism Pathway 1101!!Nitrogen Metabolism Pathway 416
ncbi summary :
can forms a synthetic lethal with cynT, rescued by high CO2. [More information is available at EcoGene: EG12319]. Escherichia coli encodes two carbonic anhydrases, Can (carbonic anhydrase 2) and CynT (carbonic anhydrase 1). [More information is available at EcoCyc: EG12319].
size :
0.1 mg
price :
310 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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