protein

VEGF121, 207-327aa, Human,His tag, Recombinant, E Coli

MBS203245

0.05 mg

375 USD

Recombinant Protein

VEGF121, 207-327aa, Human,His tag, Recombinant, E Coli

VEGF121

VPF; VEGF; VEGF-A; MGC70609; VEGF121; Vascular endothelial growth factor A isoform f; Vascular endothelial growth factor A isoform f MVCD1; Vascular endothelial growth factor A; Vascular permeability factor; VEGF A.

vascular endothelial growth factor A isoform f; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular permeability factor; vascular endothelial growth factor A; Vascular permeability factor; VPF

VEGF121

VEGFA; VEGFA; VPF; VEGF; MVCD1; VEGF; VEGF-A; VPF

VEGFA_HUMAN

327

MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

> 90% by SDS PAGE

Liquid in 20mM Tris pH 8.0 containing 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Cell line: NIH-3T3 (Mus mosculus/ embryo) . Maintenance Condition: RPMI 1640 containing 10% FBS . Assay Medium: serum free RPMI 1640 . Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Serum Free Starvation: 24hr with RPMI1640 . Incubation Time: 24 hr (after sample treatment) . Concentration Range: 100 pg/ml - 10 ug/ml . Detection method: Brdu assay

Cancer

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Yasuji ueda., et al (2004) Cancer Chemother Pharmacol. 54(1):16-24. Neufeld G., et all(1999) FASEB J. 13(1):9-22.

76781485

NP_001020541

NM_001025370.2

16.3 kDa (142aa), confirmed by MALDI-TOF.

Angiogenesis Pathway (198772); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691)

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

0.05 mg

375 USD

0.25 mg

925