protein

Gankyrin, 1-226aa, Human,Recombinant, E Coli

MBS203243

0.1 mg

310 USD

Recombinant Protein

Gankyrin, 1-226aa, Human,Recombinant, E Coli

Gankyrin

proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1; ankyrin repeat protein p28; Gankyrin; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; PSMD10.

26S proteasome non-ATPase regulatory subunit 10 isoform 1; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; gankyrin; hepatocellular carcinoma-associated protein p28-II; proteasome (prosome, macropain) 26S subunit, non-ATPase, 10; 26S proteasome regulatory subunit p28; Gankyrin; p28(GANK)

PSMD10; PSMD10; p28; p28(GANK); dJ889N15.2

PSD10_HUMAN

226

MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG

> 95% by SDS - PAGE

Liquid in Phosphate buffered saline (pH7.4), 10% glycerol Theoretical Molecular Weight: 24.4kDa (226aa)

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli

Cancer

Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. Gankyrin consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human Gankyrin was expressed in E.coli and purified by conventional chromatography techniques.

umemura A., et al. (2008) Hepatology. 47(2):493-502. Chen Y., et al. (2007) Cell Res. 17(12): 1020-9.

4506217

NP_002805

NM_002814.3

24.4kDa (226aa), confirmed by MALDI-TOF.

AMER1 Mutants Destabilize The Destruction Complex Pathway 1127621!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1127616!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1127617!!APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Securin Pathway 105833!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway 105835!!APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway 1127549!!AUF1 (hnRNP D0) Destabilizes MRNA Pathway 187212!!AXIN Missense Mutants Destabilize The Destruction Complex Pathway 1127620

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

0.1 mg

310 USD