FGF-21, 29-209aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203232 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

FGF-21, 29-209aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203232

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203232

products type :

Recombinant Protein

products full name :

FGF-21, 29-209aa, Human, His-tag, Recombinant, E Coli

products short name :

FGF-21

products name syn :

Fibroblast growth factor 21; Fibroblast growth factor 21; FGF21; Fibroblast Growth Factor-21

other names :

fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21

products gene name :

FGF-21

other gene names :

FGF21; FGF21; FGF-21

uniprot entry name :

FGF21_HUMAN

sequence length :

209

sequence :

MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: The ED50 for this effect is 83,000 units/ml). Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) . 2. Maintenance Condition: RPMI 1640 containing 10% FBS . 3. Assay Medium: serum free RPMI 1640 . 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) . 5. Serum Free Starvation: 24hr with RPMI1640 . 6. Incubation Time: 24 hr (after sample treatment) . 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution) . 8. Detection method: Brdu assay

products categories :

Hormones & Cytokines

products description :

FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. Recombinant human FGF-21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Dostalova I., et al. (2009) Physiol Res. 58(1):1-7. Kharitonenkov A., et al. (2008) BioDrugs. 22(1):37-44.

ncbi gi num :

9506597

ncbi acc num :

NP_061986

ncbi gb acc num :

NM_019113.2

ncbi mol weight :

21.6 kDa (202 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)

ncbi pathways :

MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Melanoma Pathway (83114); Melanoma Pathway (526); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 19q13.33. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage