protein

BMP- 4 (monomer), 293-408aa, Human, Recombinant, E Coli

MBS203228

0.1 mg

255 USD

Recombinant Protein

BMP- 4 (monomer), 293-408aa, Human, Recombinant, E Coli

BMP- 4

BMP-2B; DVR4; Bone morphogenetic protein 4 BMP 4; BMP2B; BMP2B1; ZYME; BMP4

bone morphogenetic protein 4 preproprotein; Bone morphogenetic protein 4; bone morphogenetic protein 4; BMP-2B; BMP-4; bone morphogenetic protein 2B; bone morphogenetic protein 4; Bone morphogenetic protein 2B; BMP-2B

BMP- 4

BMP4; BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; BMP2B; DVR4; BMP-4; BMP-2B

BMP4_HUMAN

408

MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

> 95% by SDS - PAGE

Liquid. In 10mM Sodium citrate buffer (pH 3.5), 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Hormones & Cytokines

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF-beta superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Moon BS., et al. (2009) Exp Mol Med. 41(2):116-25. Li BC., et al. (2009) J Trauma. 66(2):450-6.

157276593

NP_001193

NM_001202.3

13.2 kDa (117 aa), confirmed by MALDI-TOF.

Adipogenesis Pathway (198832); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cardiac Progenitor Differentiation Pathway (712094); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Glypican 3 Network Pathway (138084); Heart Development Pathway (198802)

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q22-q23. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity. Biological Process: negative regulation of MAP kinase activity; renal system process; extracellular matrix organization and biogenesis; macrophage differentiation; activation of MAPKK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; lymphoid progenitor cell differentiation; telencephalon regionalization; germ cell development; post-embryonic development; regulation of protein import into nucleus; BMP signaling pathway; positive regulation of endothelial cell differentiation; positive chemotaxis; chondrocyte differentiation; erythrocyte differentiation; mesonephros development; kidney development; endochondral ossification; regulation of odontogenesis of dentine-containing teeth; negative regulation of immature T cell proliferation in the thymus; positive regulation of cardiac muscle fiber development; specification of organ position; monocyte differentiation; neuron fate commitment; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; negative regulation of mitosis; positive regulation of epidermal cell differentiation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; hemopoietic progenitor cell differentiation; positive regulation of endothelial cell proliferation; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; cloacal septation; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of cell cycle; odontogenesis; negative regulation of cell proliferation; smooth muscle development; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; regulation of smooth muscle cell differentiation; dorsoventral neural tube patterning; positive regulation of BMP signaling pathway; negative regulation of epithelial cell proliferation; smoothened signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of osteoblast differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; pituitary gland development; ureteric bud branching; neural tube closure; regulation of cell fate commitment; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; positive regulation of neuron differentiation; mesodermal cell fate determination; anterior/posterior axis specification. Disease: Orofacial Cleft 11; Microphthalmia, Syndromic 6

0.1 mg

255 USD

0.5 mg

535