eIF5A, 1-154aa, Human, Recombinant, E Coli | MyBioSource MBS203210 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

eIF5A, 1-154aa, Human, Recombinant, E Coli

catalog :

MBS203210

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203210

products type :

Recombinant Protein

products full name :

eIF5A, 1-154aa, Human, Recombinant, E Coli

products short name :

eIF5A

products name syn :

EIF-5A; EIF5A1; eIF5AI; Eukaryotic translation initiation factor 5A isoform B eIF 4D; EIF 5A; eIF 5A 1; eIF 5A1; eIF4D; uORF; uORF A; Eukaryotic initiation factor 5A; Rev binding factor; Eukaryotic initiation factor 5A isoform 1; MGC99547; Eukaryotic translation initiation factor 5A; Eukaryotic translation initiation factor 5A 1; MGC104255.

other names :

eukaryotic translation initiation factor 5A-1 isoform B; Eukaryotic translation initiation factor 5A-1; eukaryotic translation initiation factor 5A-1; eIF-4D; eIF-5A-1; eIF-5A1; eukaryotic initiation factor 5A; rev-binding factor; eukaryotic translation initiation factor 5A; Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D

products gene name :

eIF5A

other gene names :

EIF5A; EIF5A; EIF-5A; EIF5A1; eIF5AI; eIF-5A-1; eIF-5A1; eIF-5A

uniprot entry name :

IF5A1_HUMAN

sequence length :

154

sequence :

MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK

purity :

> 95% by SDS - PAGE

form :

Liquid. In 50 mM Tris-HCl buffer pH 7.5 containing 10 % glycerol

concentration :

1.0 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli

products categories :

Cell Cycle

products description :

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.

products references :

Saini P, et al. (2009). Nature. 459(7243):118-21. Gosslau A., et al. (2009). J Cell Physiol. 219(2):485-93.

ncbi gi num :

4503545

ncbi acc num :

NP_001961

ncbi gb acc num :

NM_001970.4

ncbi mol weight :

16.8 kDa (154 aa), confirmed by MALDI-TOF.

ncbi pathways :

Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (106231); Hypusine Synthesis From EIF5A-lysine Pathway (106240); Metabolism Of Proteins Pathway (106230); Post-translational Protein Modification Pathway (161012); Translation Factors Pathway (198803)

uniprot summary :

EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Translation; Translation initiation. Chromosomal Location of Human Ortholog: 17p13-p12. Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; cytosol; nucleus; annulate lamellae. Molecular Function: protein binding; RNA binding; ribosome binding; protein N-terminus binding; U6 snRNA binding; translation elongation factor activity. Biological Process: mRNA export from nucleus; translational frameshifting; cellular protein metabolic process; apoptosis; positive regulation of cell proliferation; hypusine biosynthetic process from peptidyl-lysine; positive regulation of translational elongation; nucleocytoplasmic transport; protein export from nucleus; post-translational protein modification; positive regulation of translational termination

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage