Elongin B, 1-118aa, Human, Recombinant, E Coli | MyBioSource MBS203206 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Elongin B, 1-118aa, Human, Recombinant, E Coli

catalog :

MBS203206

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203206

products type :

Recombinant Protein

products full name :

Elongin B, 1-118aa, Human, Recombinant, E Coli

products short name :

Elongin B

products name syn :

Elongin B; Transcription elongation factor B polypeptide 2; TCEB2; RNA polymerase II transcription factor SIII subunit B; SIII p18; EloB; Elongin 18 kDa subunit; EloB; TCEB 2; Transcription elongation factor B (SIII) polypeptide 2.

other names :

transcription elongation factor B polypeptide 2 isoform a; Transcription elongation factor B polypeptide 2; transcription elongation factor B polypeptide 2; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; elongin 18 kDa subunit; elongin, 18-kD subunit; elongin-B; transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Elongin 18 kDa subunit; Elongin-B; EloB; RNA polymerase II transcription factor SIII subunit B; SIII p18

other gene names :

TCEB2; TCEB2; ELOB; SIII; EloB

uniprot entry name :

ELOB_HUMAN

sequence length :

118

sequence :

MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mMDTT, 0.1mM PMSF, 20% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli

products categories :

Cell Cycle

products description :

Elongin B, also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

products references :

Piessevaux J., et al. (2008) J Biol Chem. 283(31):21334-46. Duan DR., et al. (1995) Science. 269(5229):1402-6.

ncbi gi num :

6005890

ncbi acc num :

NP_009039

ncbi gb acc num :

NM_007108.3

ncbi mol weight :

13.1 kDa (118 aa), confirmed by MALDI-TOF.

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Disease Pathway (530764); ECS Complex Pathway (413455); ECS Complex Pathway (468394); ECV Complex Pathway (413450); ECV Complex Pathway (890606)

ncbi summary :

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]

uniprot summary :

TCEB2: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Chromosomal Location of Human Ortholog: 16p12.3. Cellular Component: nucleoplasm; Cul2-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex; cytosol. Molecular Function: protein binding; ubiquitin protein ligase binding. Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; protein ubiquitination; protein complex assembly; gene expression

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage