protein

GADD153,1-169aa, Human, His-tag, Recombinant, E Coli

MBS203198

0.1 mg

375 USD

Recombinant Protein

GADD153,1-169aa, Human, His-tag, Recombinant, E Coli

GADD153

CHOP; DDIT3; CEBPZ; CHOP10; MGC4154; Growth arrest-and DNA damage-inducible gene 153; GADD153; Growth arrest-and DNA damage-inducible gene 153 C/EBP homologous protein; C/EBP Homology Protein; CHOP 10; DDIT 3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153

DNA damage-inducible transcript 3 protein isoform 2; DNA damage-inducible transcript 3 protein; DNA damage-inducible transcript 3 protein; C/EBP zeta; CCAAT/enhancer-binding protein homologous protein; DDIT-3; c/EBP-homologous protein 10; growth arrest and DNA damage-inducible protein GADD153; DNA-damage-inducible transcript 3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153

GADD153

DDIT3; DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10

DDIT3_HUMAN

169

MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

> 90% by SDS - PAGE

Liquid in 20mM Tris-HCl buffer pH8.0 containing 20% Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag. Category: Primary antibody

Expression System: E Coli

Cell Cycle

GADD153, also known as DNA-damage-inducible transcript 3 (DDIT3), is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. This protein has been shown to be up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. GADD153 protein may play a role in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. Recombinant GADD153 fused with His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

Oyadomari S. and Mori M., et al.(2004) Cell death and differentiation,11: 381-389. Robert M. Silva.,et al:(2005) Journal of Neurochemistry ;95(4):974-986.

21361118

NP_004074

NM_004083.5

21.3kDa (189aa), confirmed by MALDI-TOF.

ATF-2 Transcription Factor Network Pathway (138006); ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adipogenesis Pathway (198832); MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Metabolism Of Proteins Pathway (106230); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188)

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform. Protein type: Autophagy; DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 12q13.1-q13.2. Cellular Component: nucleoplasm; late endosome; nucleus; cytosol. Molecular Function: protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; transcription factor activity; transcription corepressor activity; transcription factor binding. Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; inhibition of CREB transcription factor; response to DNA damage stimulus. Disease: Myxoid Liposarcoma

0.1 mg

375 USD

0.5 mg

925