catalog number :
MBS203197
products type :
Recombinant Protein
products full name :
Ferric uptake regulator(FuR), 1-148aa E Coli, Recombinant, E Coli
products short name :
Ferric uptake regulator
products name syn :
DNA-binding transcriptional dual regulator of siderophore biosynthesis and transport; Ferric uptake regulator; FuR; ECK0671; Ferric uptake regulation protein; JW0669.
other names :
ferric iron uptake regulon transcriptional repressor; autorepressor; Ferric uptake regulation protein; ferric iron uptake regulon transcriptional repressor; autorepressor
other gene names :
fur; fur; ECK0671; JW0669; Ferric uptake regulator
uniprot entry name :
FUR_ECOLI
sequence :
MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK
purity :
> 95% by SDS - PAGE
form :
Liquid in 20mM Tris pH 8.0, 2mM CaCl2, 100mM NaCl
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human
other info2 :
Expression System: E Coli
products categories :
Cell Cycle
products description :
Fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. Fur is a small, 17-kDa, global transcriptional repressor that in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the Fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB. Recombinant FuR was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
O'Sullivan DJ., et al. (1994). FEMS Microbiol Lett. Apr 15; 117(3):327-32. Mills SA., et al. (2005) Biochemistry. Oct 18; 44(41):13553-9.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
16.7kDa (148aa), confirmed by MALDI-TOF.
ncbi summary :
Fur translation in coupled to the translation of the upstream leader pepidie Uof; Uof translation is inhibited by the antisense sRNA ryhB; Uof translation opens up a hairpin that blocks Fur translation (Vecerek, 2007). [More information is available at EcoGene: EG10359].