protein

FKBP4, 1-459aa, Human, His-tag, Recombinant, E Coli

MBS203196

0.1 mg

255 USD

Recombinant Protein

FKBP4, 1-459aa, Human, His-tag, Recombinant, E Coli

FKBP4

FKBP52; FKBP59; HBI; Hsp56; p52; PPIase; FK506 binding protein 4 52 kDa FK506 binding protein; FKBP 4; FKBP 52; FKBP 59; FKBP4; FKBP52 protein; FKBP59; Hsp 56; HSP binding immunophilin; p59 protein; PPIase; Rotamase; Peptidyl prolyl cis trans isomerase; Peptidylprolyl cis trans isomerase; T cell FK506 binding protein 59kD.

peptidyl-prolyl cis-trans isomerase FKBP4; Peptidyl-prolyl cis-trans isomerase FKBP4; peptidyl-prolyl cis-trans isomerase FKBP4; 51 kDa FK506-binding protein; FK506-binding protein 4 (59kD); HSP binding immunophilin; T-cell FK506-binding protein, 59kD; peptidylprolyl cis-trans isomerase; rotamase; FK506 binding protein 4, 59kDa; 51 kDa FK506-binding protein; FKBP51; 52 kDa FK506-binding protein; 52 kDa FKBP; FKBP-52; 59 kDa immunophilin; p59; FK506-binding protein 4; FKBP-4; FKBP59; HSP-binding immunophilin; HBI; Immunophilin FKBP52; RotamasePeptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed

FKBP4

FKBP4; FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase; FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI

FKBP4_HUMAN

459

MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl (pH8.0) buffer containing 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH 8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant FKBP4 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

Heat Shock Proteins

FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. Recombinant human FKBP4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Ostrow KL., et al. (2009) Clin Cancer Res. 15(4):1184-91. Ma D., et al. (2008) J Biol Chem. 283(38):25963-70.

4503729

NP_002005

NM_002014.3

53.9 kDa (479 aa), confirmed by MALDI-TOF.

Androgen Receptor Signaling Pathway 198806!!Attenuation Phase Pathway 1127556!!Cellular Response To Heat Stress Pathway 1127553!!Cellular Responses To Stress Pathway 645258!!Coregulation Of Androgen Receptor Activity Pathway 138085!!Estrogen Signaling Pathway 799177!!Estrogen Signaling Pathway 799197!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!HSF1-dependent Transactivation Pathway 1127555

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]

0.1 mg

255 USD