protein

FKBP1a /FKBP12, 1-108aa, Human, His-tag, Recombinant, E Coli

MBS203195

0.1 mg

285 USD

Recombinant Protein

FKBP1a /FKBP12, 1-108aa, Human, His-tag, Recombinant, E Coli

FKBP1a /FKBP12

PPIase FKBP1A; Rotamase; FKBP1; PKC12; FK506 binding protein 1a; 12kDa 12 kDa FKBP; FK506 binding protein 1; FK506 binding protein 1A 12kDa; Immunophilin FKBP12; FK506 binding protein T cell 12 kD; FK506-binding protein 1A; FK506 binding protein 12; FKBP 12; FKBP 1A; FKBP12; FKBP12 Exip3; FKBP12C; Peptidyl-prolyl cis-trans isomerase; PKCI2; PPIase; Protein kinase C inhibitor 2.

peptidyl-prolyl cis-trans isomerase FKBP1A isoform a; Peptidyl-prolyl cis-trans isomerase FKBP1A; peptidyl-prolyl cis-trans isomerase FKBP1A; 12 kDa FK506-binding protein; 12 kDa FKBP; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD; FKBP12-Exip3; PPIase FKBP1A; calstabin 1; calstabin-1; immunophilin FKBP12; protein kinase C inhibitor 2; rotamase; FK506 binding protein 1A, 12kDa; 12 kDa FK506-binding protein; 12 kDa FKBP; FKBP-12; Calstabin-1; FK506-binding protein 1A; FKBP-1A; Immunophilin FKBP12; Rotamase

FKBP1a /FKBP12

FKBP1A; FKBP1A; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP-1A; FKBP1; FKBP12; PPIase FKBP1A; 12 kDa FKBP; FKBP-12; FKBP-1A

FKB1A_HUMAN

108

MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH 8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10ul of recombinant FKBP1a/FKBP12 protein with 1ug in assay buffer. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). Record the increase in A405 nm for 30 minutes at 25C.

Heat Shock Proteins

FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Jayaraman T., et al. (1992). J Biol Chem. 267(14):9474-7. Siekierka JJ., et al. (1990). J Biol Chem. 265(34):21011-5.

17149836

NP_463460

NM_054014.3

14.1 kDa (128aa), confirmed by MALDI-TOF.

ALK1 Pathway (137985); ALK1 Signaling Events Pathway (137968); ALK2 Signaling Events Pathway (137983); Alpha-synuclein Signaling Pathway (137913); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling In The CD4+ TCR Pathway (137941); Disease Pathway (530764); Integrated Pancreatic Cancer Pathway (711360); Loss Of Function Of SMAD2/3 In Cancer Pathway (906019); Loss Of Function Of SMAD4 In Cancer Pathway (906017)

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium. Multiple alternatively spliced variants, encoding the same protein, have been identified. The human genome contains five pseudogenes related to this gene, at least one of which is transcribed. [provided by RefSeq, Sep 2008]

FKBP12: an immunophilin and peptidyl-prolyl cis-trans isomerase that binds to the immunosuppressant drugs FK506 (a.k.a. tacrolimus or Fujimycin) and rapamycin (a.k.a. Sirolimus). The FKBP12/rapamycin complex binds NFAT transcription factors and inhibits the induction of T cell genes including IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. FK506 is used in treating patients after organ transplant and those suffering from autoimmune disorders. The immunosuppressant activity of FKBP12 is not apparently related to its prolyl isomerase activity. The FKBP12/rapamycin complex inhibits the mammalian target of rapamycin (mTOR) pathway by directly binding the mTOR Complex1 (mTORC1). mTORC1 contains Raptor, mLST8, and PRAS40, and interacts with DEPTOR, which inhibits its activity. mTOR, a S/T protein kinase, is a central regulator of cellular growth and metabolism. Its inhibition enhances the dependence on aerobic glycolysis in leukemic cells. Protein type: EC 5.2.1.8; Isomerase. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; cytoplasm; nerve terminal; Z disc; cytosol. Molecular Function: protein binding; signal transducer activity; peptidyl-prolyl cis-trans isomerase activity; enzyme binding; protein homodimerization activity; FK506 binding; activin binding; macrolide binding; Hsp70 protein binding; calcium channel inhibitor activity; SMAD binding; transforming growth factor beta receptor binding. Biological Process: heart morphogenesis; protein maturation via protein folding; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein peptidyl-prolyl isomerization; T cell activation; positive regulation of protein binding; protein folding; cytokine and chemokine mediated signaling pathway; regulation of activin receptor signaling pathway; SMAD protein complex assembly; response to caffeine; negative regulation of protein phosphatase type 2B activity; T cell proliferation; de novo protein folding; regulation of protein localization; muscle contraction; negative regulation of protein amino acid phosphorylation; positive regulation of protein ubiquitination; negative regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; transforming growth factor beta receptor signaling pathway; fibril organization and biogenesis; ventricular cardiac muscle morphogenesis; protein refolding; response to iron ion

0.1 mg

285 USD

0.5 mg

640