catalog number :
MBS203194
products type :
Recombinant Protein
products full name :
DsbE, 26-185aa, E Coli, Recombinant, E Coli
products short name :
DsbE
products name syn :
CcmG; yejQ; Thiol:disulfide interchange protein dsbE Cytochrome c biogenesis protein ccmG. ECK2187; Periplasmic thioredoxin of cytochrome c type biogenesis; JW2183.
other names :
periplasmic thioredoxin of cytochrome c-type biogenesis; Thiol:disulfide interchange protein DsbE; periplasmic thioredoxin of cytochrome c-type biogenesis; Cytochrome c biogenesis protein CcmG
products gene name :
DsbE
other gene names :
dsbE; dsbE; ccmG; ECK2187; JW2183; yejQ; ccmG; yejQ
uniprot entry name :
DSBE_ECOLI
sequence :
MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 2 mM EDTA 10% glycerol.
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E.coli and purified by using conventional chromatography.
products references :
Reid E., et al. (2001). Biochem J. 355(1):51-8. Li Q., et al. (2001). Biol Chem. 382(12):1679-86.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
18.1 kDa (161aa), confirmed by MALDI-TOF.
ncbi summary :
CcmG (dsbE) is located in periplasm, anchored to membrane. [More information is available at EcoGene: EG12053]. CcmG is a membrane protein component of the CcmEFGH holocytochrome c synthetase. [More information is available at EcoCyc: EG12053].