catalog number :
MBS203193
products type :
Recombinant Protein
products full name :
DsbG, 18-248aa, E Coli, Recombinant, E Coli
products short name :
DsbG
products name syn :
ybdP; Thiol:disulfide interchange protein dsbG ECK0598; JW0597; Periplasmic disulfide isomerase/thiol disulphide oxidase
other names :
thiol:disulfide interchange protein, periplasmic; Thiol:disulfide interchange protein DsbG; thiol:disulfide interchange protein, periplasmic
products gene name :
DsbG
other gene names :
dsbG; dsbG; ECK0598; JW0597; ybdP; ybdP
uniprot entry name :
DSBG_ECOLI
sequence :
MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E.coli and purified by conventional chromatography techniques.
products references :
Riley M., et al (2006) Nucleic Acids Res. 34(1):1-9. Andersen CL., et al (1997) Mol Microbiol. 26(1):121-32.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
25.8 kDa(232 aa), confirmed by MALDI-TOF.
ncbi summary :
A dsbG defect iscorrected by DsbAB overexpression. [More information is available at EcoGene: EG13535].