protein

HSP90B1(GRP94), 22-803aa, Human, His-tag, Recombinant, E Coli

MBS203192

0.1 mg

510 USD

Recombinant Protein

HSP90B1(GRP94), 22-803aa, Human, His-tag, Recombinant, E Coli

HSP90B1

94 kDa glucose-regulated protein; HSP90B1; GP96; TRA1; Tumor rejection antigen 1; GRP94; 94 kDa glucose-regulated protein 94 kDa glucose regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 9; Heat shock protein 90 kDa beta member 1; Stress inducible tumor rejection antigen GP96; Tumor rejection antigen gp96.

endoplasmin; Endoplasmin; endoplasmin; 94 kDa glucose-regulated protein; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96; tumor rejection antigen (gp96) 1; tumor rejection antigen 1; heat shock protein 90kDa beta (Grp94), member 1; 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog

HSP90B1

HSP90B1; HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m; GRP94; TRA1; GRP-94

ENPL_HUMAN

803

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV GTDEEEETAK ESTAEKDEL

> 90% by SDS-PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1mM EDTA, 0.1M NaCl, 10% glycerol,

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Heat Shock Proteins

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Recombinant human GRP94 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Randow F., et al. (2001). Nat Cell Biol. 3(10):E231. Li Z., et al. (2002). Front Biosci. 7:d731-51.

4507677

NP_003290

NM_003299.2

94.4 kDa (819aa)

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); Metabolism Of Proteins Pathway (106230); NOD-like Receptor Signaling Pathway (122191)

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family. Protein type: Secreted, signal peptide; Heat shock protein; Secreted; Apoptosis. Chromosomal Location of Human Ortholog: 12q24.2-q24.3. Cellular Component: extracellular matrix; endoplasmic reticulum membrane; focal adhesion; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; extracellular region; plasma membrane; midbody; nucleus; cytosol. Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding. Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; sequestering of calcium ion; toll-like receptor signaling pathway; response to hypoxia; innate immune response; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis

0.1 mg

510 USD

0.5 mg

1335