Crystallin alpha B, 1-175aa, Mouse, Recombinant, E Coli | MyBioSource MBS203184 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Crystallin alpha B, 1-175aa, Mouse, Recombinant, E Coli

catalog :

MBS203184

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203184

products type :

Recombinant Protein

products full name :

Crystallin alpha B, 1-175aa, Mouse, Recombinant, E Coli

products short name :

Crystallin alpha B

products name syn :

Crya2; Crya-2; Cryab; Crystallin alpha B

other names :

alpha-crystallin B chain; Alpha-crystallin B chain; alpha-crystallin B chain; alpha B-crystallin; alpha(B)-crystallin; crystallin, alpha 2; heat shock protein CryAB; heat shock protein HspB5; crystallin, alpha B; Alpha(B)-crystallin; P23

other gene names :

Cryab; Cryab; P23; Crya2; HspB5; Crya-2; Crya2

uniprot entry name :

CRYAB_MOUSE

sequence length :

175

sequence :

MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK

purity :

> 95% by SDS - PAGE

form :

Liquid in 20mM Tris pH 8.0 and 10 % glycerol Molecular Weight: 20 kDa (175 aa)

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Mouse

other info2 :

Expression System: E Coli

products categories :

Heat Shock Proteins

products description :

Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Hasan, A., et al.(2002) Biochemistry 41(52) 15876-15882. Kamradt,M.C., et al.(2002) J. Biol.Chem. 277(41) 38731-38736 . Reddy, G.B., et al. (2002) FEBS lett. 522(1-3) 59-64 . Ito, H., et al. (2002) J.Biochem.131(4) 593-603.

ncbi gi num :

6753530

ncbi acc num :

NP_034094

ncbi gb acc num :

NM_009964.3

ncbi mol weight :

20kDa (175aa), confirmed by MALDI-TOF.

ncbi pathways :

Protein Processing In Endoplasmic Reticulum Pathway (167331); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

This gene encodes a member of the small heat-shock protein (HSP20) family. The encoded protein is a molecular chaperone that protects proteins against thermal denaturation and other stresses. This protein is a component of the eye lens, regulates lens differentiation and functions as a refractive element in the lens. This protein is a negative regulator of inflammation, has anti-apoptotic properties and also plays a role in the formation of muscular tissue. Mice lacking this gene exhibit worse experimental autoimmune encephalomyelitis and inflammation of the central nervous system compared to the wild type. In mouse models, this gene has a critical role in alleviating the pathology of the neurodegenerative Alexander disease. Mutations in the human gene are associated with myofibrillar myopathy 2, fatal infantile hypertonic myofibrillar myopathy, multiple types of cataract and dilated cardiomyopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Protein type: Heat shock protein; Chaperone. Cellular Component: I band; Golgi apparatus; cell surface; mitochondrion; contractile fiber; actin filament bundle; cytosol; Z disc; microtubule cytoskeleton; cytoplasm; plasma membrane; nucleus; myelin sheath. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; microtubule binding; metal ion binding; unfolded protein binding; cytoskeletal protein binding; structural constituent of eye lens. Biological Process: lens development in camera-type eye; muscle development; camera-type eye development; protein folding; microtubule polymerization or depolymerization; stress-activated MAPK cascade; negative regulation of caspase activity; tubulin folding; response to hydrogen peroxide; negative regulation of intracellular transport; response to hypoxia; negative regulation of cell growth; protein homooligomerization; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage