product summary
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company name :
MyBioSource
product type :
protein
product name :
SlyD,1-196aa, E Coli, Recombinant, E Coli
catalog :
MBS203183
quantity :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203183
products type :
Recombinant Protein
products full name :
SlyD,1-196aa, E Coli, Recombinant, E Coli
products short name :
SlyD
products name syn :
FKBP-type peptidyl-prolyl cis-trans isomerase; SlyD; FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD; Histidine rich protein; PPIase; Rotamase; WHP.
other names :
FKBP-type peptidyl-prolyl cis-trans isomerase; FKBP-type peptidyl-prolyl cis-trans isomerase SlyD; FKBP-type peptidylprolyl isomerase; Metallochaperone SlyD
products gene name :
SlyD
other gene names :
slyD; slyD; PPIase
uniprot entry name :
SLYD_ECOL6
sequence length :
196
sequence :
MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
purity :
> 95% by SDS - PAGE
form :
Liquid in 20mM Tris pH 7.5
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli. Bioactivity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant SlyD protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.
products categories :
Heat Shock Proteins
products description :
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E.Coli slyD was expressed in E.coli and purified by conventional chromatography.
products references :
Zhang JW., et al. (2007) J Bacteriol. 189(21):7942-4. Scholz C., et al. (2006) Biochemistry. 45(1):20-33.
ncbi gi num :
26249947
ncbi acc num :
NP_755987
ncbi gb acc num :
NC_004431.1
ncbi mol weight :
21 kDa (196 aa), confirmed by MALDI-TOF.
size :
0.1 mg
price :
310 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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