protein

Recombinant Thyroid Stimulating Hormone Receptor (TSHR)

MBS2031756

0.01 mg

140 USD

Recombinant Protein

Recombinant Thyroid Stimulating Hormone Receptor (TSHR)

[Thyroid Stimulating Hormone Receptor]

[thyroid stimulating hormone receptor; Thyrotropin receptor; thyrotropin receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; TSH-R]

[TSHR]

[TSHR; TSHR; LGR3; CHNG1; hTSHR-I; LGR3; TSH-R]

TSHR_HUMAN

E Coli

764

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. PCE CHQEDDFRVT CKELHQIPSLPPSTQTLKLI ETHLKTIPSL AFSSLPNISR IYLSIDATLQ RLEPHSFYNLSKMTHIEIRN TRSLTYIDPD ALTELPLLKF LGIFNTGLRI FPDLTKIYSTDVFFILEITD NPYMTSVPEN AFQGLCNETL TLKLYNNGFT SIQGHAFNGTKLDAVYLNKN KYLTAIDKDA FGGVYSGPTL LDVSSTSVTA LPSKG

>97%

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

200 ug/mL

Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE, Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression. Residues: Pro28~Gly245. Tags: N-terminal His Tag. Traits: Freeze-dried powder

Predicted isoelectric point: 5.9. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

38016895

AAR07906.1

Predicted Molecular Mass:28.1KDa. Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.

Arf6 Signaling Events Pathway (138034); Arf6 Trafficking Events Pathway (137954); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Hormone Ligand-binding Receptors Pathway (1269559); Neuroactive Ligand-receptor Interaction Pathway (83053)

The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

TSHR: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH- independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation. Defects in TSHR are the cause of congenital hypothyroidism non-goitrous type 1 (CHNG1); also known as congenital hypothyroidism due to TSH resistance. CHNG1 is a non-autoimmune condition characterized by resistance to thyroid- stimulating hormone (TSH) leading to increased levels of plasma TSH and low levels of thyroid hormone. CHNG1 presents variable severity depending on the completeness of the defect. Most patients are euthyroid and asymptomatic, with a normal sized thyroid gland. Only a subset of patients develop hypothyroidism and present a hypoplastic thyroid gland. Defects in TSHR are the cause of familial gestational hyperthyroidism (HTFG). HTFG is a condition characterized by abnormally high levels of serum thyroid hormones occurring during early pregnancy. Defects in TSHR are the cause of hyperthyroidism non- autoimmune (HTNA). It is a condition characterized by abnormally high levels of serum thyroid hormones, thyroid hyperplasia, goiter and lack of anti-thyroid antibodies. Typical features of Graves disease such as exophthalmia, myxedema, antibodies anti-TSH receptor and lymphocytic infiltration of the thyroid gland are absent. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 14q31. Cellular Component: integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: protein binding; thyroid-stimulating hormone receptor activity. Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation. Disease: Hyperthyroidism, Nonautoimmune; Hypothyroidism, Congenital, Nongoitrous, 1; Hyperthyroidism, Familial Gestational

0.01 mg

140 USD

0.05 mg

255

0.1 mg

390

0.2 mg

480

0.5 mg

935

1 mg

1380