Neuron-Specific Enolase (NSE), 1-434aa, Human, Recombinant, E Coli | MyBioSource MBS203166 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Neuron-Specific Enolase (NSE), 1-434aa, Human, Recombinant, E Coli

catalog :

MBS203166

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203166

products type :

Recombinant Protein

products full name :

Neuron-Specific Enolase (NSE), 1-434aa, Human, Recombinant, E Coli

products short name :

Neuron-Specific Enolase

products name syn :

Enolase 2 (gamma; neuronal); ENO2; NSE; Neuron-Specific Enolase; 2 phospho D glycerate hydrolyase; Eno 2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma enolase; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neurone specific enolase.

other names :

gamma-enolase; Gamma-enolase; gamma-enolase; 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; epididymis secretory protein Li 279; neural enolase; neuron specific gamma enolase; neuron-specific enolase; neurone-specific enolase; enolase 2 (gamma, neuronal); 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

products gene name :

NSE

other gene names :

ENO2; ENO2; NSE; HEL-S-279; NSE

uniprot entry name :

ENOG_HUMAN

sequence length :

434

sequence :

MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

purity :

> 95% by SDS - PAGE

form :

Liquid in 20 mM Tris pH 7.5, 100mM KCl, 5 mM MgSO4

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is < 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH6.5 at 37 degree C. Endotoxin: < 1.0 Eu per 1 ug of protein (determined by LAL method). Assay: 1. Prepare 1,450ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2-phosphorglycerate, 0.12 mM beta- NADPH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase, 6 unit L-lactic dehydrogenas. 2. Add 50ul of recombinant NSE protein in various concentrations (0.25 ug, 0.5 ug, 0.1 ug) in assay buffer. 3. Mix by inversion and load 200 ul of reaction mix in to a plate well. 4. Record the decrease in A340 nm for 5 minutes at 25C.

products categories :

Neuroscience

products description :

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

products references :

T Kirino., et al.(1983). J. Neuroscience. 3, 915-923. Johnson DH., et al.(1984). Cancer Res. 44(11):5409-14.

ncbi gi num :

5803011

ncbi acc num :

NP_001966

ncbi gb acc num :

NM_001975.2

ncbi mol weight :

47 kDa (434 aa), confirmed by MALDI-TOF.

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (1127581)

ncbi summary :

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

uniprot summary :

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: extracellular space; photoreceptor inner segment; phosphopyruvate hydratase complex; plasma membrane; perikaryon; cytosol. Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity. Biological Process: glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage