catalog number :
MBS203163
products type :
Recombinant Protein
products full name :
His-Streptavidin, 25-183aa, Streptomyces avidinii, His tag, Recombinant, E Coli
products short name :
His-Streptavidin
products name syn :
Streptavidin; Streptomyces avidinii streptavidin; SA V1; SA V2; Streptavidin V1; Streptavidin V2.
other names :
unnamed protein product; Streptavidin
uniprot entry name :
SAV_STRAV
sequence :
MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ
purity :
> 95% by SDS - PAGE
form :
Liquid in 20mM Tris pH 7.5
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Streptomyces avidinii. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Fusion Tag & Detection
products description :
Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Howarth M., et al. (2006) Nat Methods. 3(4):267-73. Holmberg A., et al (2005). Electrophoresis. 26(3):501-10.
ncbi mol weight :
17 kDa (167 aa), confirmed by MALDI-TOF.
uniprot summary :
streptavidin: The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). (organism: Streptomyces avidinii)