protein

Recombinant F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

MBS2031603

0.01 mg

145 USD

Recombinant Protein

Recombinant F-Box And WD Repeat Domain Containing Protein 7 (FBXW7)

[F-Box And WD Repeat Domain Containing Protein 7]

[F-box and WD repeat domain containing 7; F-box/WD repeat-containing protein 7; F-box/WD repeat-containing protein 7; F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase; Archipelago homolog]

[FBXW7]

[FBXW7; FBXW7; AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10; hAgo]

FBXW7_HUMAN

Host: E Coli

Homo sapiens (Human)

707

>95%

Original concentration: 200 Ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

. Source: Prokaryotic expression . Buffer: 100 mMNaHCO 3 ,500 mMNaCI, pH8.3, containing 0.01% SKL,5% Trehalose and Proclin300. Predicted isoelectric point: 9.3. Predicted Molecular Mass: 30.8kDa. Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.

. Tags: N-terminal His Tag. Organism Species: Homo sapiens (Human). Residues: Asp279~Val515. Subcellular location: Nucleus. Traits: Freeze dried powder . Usage: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

109659002

AAI17247.1

Adaptive Immune System Pathway (1269171); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (1269193); Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway (1268699); Chaperonin-mediated Protein Folding Pathway (1268694); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1268891); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1268889); Delta-Notch Signaling Pathway (198879); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855)

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

FBXW7: a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins including MYC, cyclin E, NOTCH1-released notch intracellular domain (NICD), JUN, and probably PSEN1. Recognizes and binds to phosphorylated target proteins. Other components of the SCF(FBXW7) complex are sCUL1, RBX1, and SKP1A. Defects in FBXW7 may be a cause of breast cancer. The homolog of Cdc4, one of the yeast genes that regulate sister chromatid cohesion. Down-regulation or disruption of FBXW7 resulted in abnormal sister chromatid cohesion in human cells. Four alternatively spliced isoforms have been described. Protein type: Nucleolus; Ubiquitin conjugating system; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 4q31.3. Cellular Component: nucleoplasm; Golgi apparatus; protein complex; endoplasmic reticulum; cytoplasm; nucleolus; SCF ubiquitin ligase complex. Molecular Function: protein binding, bridging; identical protein binding; cyclin binding; protein binding; ubiquitin protein ligase binding; transcription factor activity. Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; protein stabilization; viral reproduction; sister chromatid cohesion; lipid homeostasis; negative regulation of DNA endoreduplication; protein ubiquitination; regulation of protein localization; vasculature development; positive regulation of epidermal growth factor receptor activity; regulation of transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; negative regulation of Notch signaling pathway; vasculogenesis; response to DNA damage stimulus; lung development

0.01 mg

145 USD

0.05 mg

275

0.1 mg

430

0.2 mg

525

0.5 mg

1015

1 mg

1515