protein

Recombinant Toll Like Receptor 9 (TLR9)

MBS2031580

0.01 mg

140 USD

Recombinant Protein

Recombinant Toll Like Receptor 9 (TLR9)

[Toll Like Receptor 9]

[toll-like receptor 9; Toll-like receptor 9; toll-like receptor 9; toll-like receptor 9; CD_antigen: CD289]

[TLR9]

[Tlr9; Tlr9]

TLR9_MOUSE

E. coli

> 97%

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 50 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Testing Data

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Thr28~Ser362. Tags: N-terminal His Tag. Subcellular Location: Lysosome, Cytoplasm, Endoplasmic reticulum lumen, Endosome. Traits: Freeze-dried powder

Predicted isoelectric point: 9.1. Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

157057166

NP_112455.2

NM_031178.2

Predicted Molecular Mass: 41.1kDa. Accurate Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions.

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); IGF1R Signaling Cascade Pathway (1254242); IRS-mediated Signalling Pathway (1254055); IRS-related Events Triggered By IGF1R Pathway (1254244); Immune System Pathway (1254661)

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Cellular Component: membrane; basolateral plasma membrane; endoplasmic reticulum; lysosome; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; endosome. Molecular Function: protein binding; transmembrane receptor activity; siRNA binding; interleukin-1 receptor binding; unmethylated CpG binding. Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; regulation of B cell activation; positive regulation of NF-kappaB import into nucleus; signal transduction; positive regulation of interleukin-18 production; defense response to Gram-negative bacterium; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; positive regulation of interferon-beta production; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; inflammatory response; defense response to virus; positive regulation of autophagy; regulation of protein amino acid phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; positive regulation of interferon-beta biosynthetic process; negative regulation of toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process

0.01 mg

140 USD

0.05 mg

260

0.1 mg

400

0.2 mg

495

0.5 mg

955

1 mg

1415