Livin, 1-298aa, Human, His tag, E Coli | MyBioSource MBS203155 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Livin, 1-298aa, Human, His tag, E Coli

catalog :

MBS203155

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS203155

products type :

Recombinant Protein

products full name :

Livin, 1-298aa, Human, His tag, E Coli

products short name :

[Livin]

products name syn :

[Livin inhibitor of apoptosis isoform alpha; BIRC7; Baculoviral IAP repeat-containing 7; KIAP; MLIAP; RNF50; ML-IAP; KIAP; MLIAP; RNF50; Livin; Livin inhibitor of apoptosis isoform alpha; Livin inhibitor of apoptosis isoform alpha Baculoviral IAP repeat containing 7; Baculoviral IAP repeat containing protein 7; BIRC 7; Kidney inhibitor of apoptosis protein; Livin inhibitor of apotosis; RING finger protein 50; RNF 50; Melanoma inhibitor of apoptosis protein; ML IAP.]

other names :

[baculoviral IAP repeat-containing protein 7 isoform alpha; Baculoviral IAP repeat-containing protein 7; baculoviral IAP repeat-containing protein 7; baculoviral IAP repeat containing 7; Kidney inhibitor of apoptosis protein; KIAP]

other gene names :

[BIRC7; BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP; KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin]

host :

E Coli

sequence length :

298

sequence :

MGSSHHHHHH SSGLVPRGSH MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS

purity :

> 90% by SDS-PAGE

form :

Liquid in 20mM Tris pH 8.0, 1mM DTT, 0.1M NaCl, 2mM EDTA, 20% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

other info1 :

Antigen Species: Human. Tag Information: His-tag

products categories :

Cancer

products description :

Livin is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin fused to His-tag at N-terminus was expressed in E Coli and purified by conventional chromatography techniques.

products references :

Kasof GM., et al. (2001). J Biol Chem. 276 (5):3238-46.; Ashhab Y., et al. (2001). FEBS Lett. 495 (1-2):56-60.;

ncbi gi num :

21536421

ncbi acc num :

NP_647478

ncbi gb acc num :

NM_139317.2

uniprot acc num :

Q96CA5

ncbi mol weight :

34 kDa (318 aa), confirmed by MALDI-TOF.

ncbi pathways :

Apoptosis - Multiple Species Pathway (1384747); Apoptosis - Multiple Species Pathway (1384709); Apoptosis Modulation And Signaling Pathway (198822); Pathways In Cancer (83105); Small Cell Lung Cancer Pathway (83118); Small Cell Lung Cancer Pathway (530); Toxoplasmosis Pathway (169642); Toxoplasmosis Pathway (169493); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

uniprot summary :

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage