HMGB1, 1-215aa, Human, His tag, Recombinant, Baculovirus | MyBioSource MBS203153 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

HMGB1, 1-215aa, Human, His tag, Recombinant, Baculovirus

catalog :

MBS203153

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203153

products type :

Recombinant Protein

products full name :

HMGB1, 1-215aa, Human, His tag, Recombinant, Baculovirus

products short name :

HMGB1

products name syn :

HMG1; HMG3; SBP-1; High-mobility group box1; HMGB1; High-mobility group box1; high-mobility group (nonhistone chromosomal) protein 1; Sulfoglucuronyl carbohydrate binding protein; Amphoterin; high mobility group protein 1; DKFZp686A04236

other names :

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high mobility group box 1; High mobility group protein 1; HMG-1

products gene name :

HMGB1

other gene names :

HMGB1; HMGB1; HMG1; HMG3; SBP-1; HMG1; HMG-1

uniprot entry name :

HMGB1_HUMAN

sequence length :

215

sequence :

MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

purity :

> 90% by SDS - PAGE

form :

Liquid in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: Hi-5. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Hormones & Cytokines

products description :

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.

products references :

Palumbo R J., et al. (2004) Cell Biol. 164(3):441-9. Andersson u., et al (2002) Leukoc Biol. 72(6):1084-91.

ncbi gi num :

4504425

ncbi acc num :

NP_002119

ncbi gb acc num :

NM_002128.4

ncbi mol weight :

25kDa (223aa), confirmed by MALDI-TOF.

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Activation Of DNA Fragmentation Factor Pathway (105683); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Apoptosis Pathway (105648); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822)

uniprot summary :

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Protein type: Nuclear receptor co-regulator; DNA repair, damage. Chromosomal Location of Human Ortholog: 13q12. Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus. Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; DNA bending activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity. Biological Process: negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; DNA topological change; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; DNA ligation during DNA repair; innate immune response; dendritic cell chemotaxis; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; positive regulation of DNA binding; cell structure disassembly during apoptosis

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage