protein

Cyclophilin D(PPID), 1- 370aa, Human, His-tag, Recombinant, E Coli

MBS203148

0.1 mg

375 USD

Recombinant Protein

Cyclophilin D(PPID), 1- 370aa, Human, His-tag, Recombinant, E Coli

Cyclophilin D

Peptidylprolyl isomerase D; PPID; CYPD; CYP-40; Cyclophilin D; 40 kDa peptidyl prolyl cis trans isomerase; 40 kDa peptidyl prolyl cis trans isomerase D; Cyclophilin related protein; Cyclophilin40; CyclophilinD; CYP 40; CYP40; MGC33096.Peptidylprolyl isomerase D; PPIase; PPID; Rotamase.

peptidyl-prolyl cis-trans isomerase D; Peptidyl-prolyl cis-trans isomerase D; peptidyl-prolyl cis-trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase D; PPIase D; cyclophilin 40; cyclophilin D; cyclophilin-40; cyclophilin-related protein; rotamase D; peptidylprolyl isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin-40; CYP-40; Cyclophilin-related protein; Rotamase D

PPID; PPID; CYPD; CYP-40; CYP40; CYPD; PPIase D; CYP-40

PPID_HUMAN

370

MGSSHHHHHH SSGLVPRGSH MSHPSPQAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQP IALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGL KEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA

> 95% by SDS - PAGE

Liquid in Phosphate buffered saline (pH7.4), 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 210 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant Cyclophilin D protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM). 5. Record the increase in A405 nm for 30 minutes at 25C.

Cell Cycle

Cyclophilin D (also known as peptidylpropyl isomerase D, PPID) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression suppresses the apoptosis in cancer cells. Recombinant human cyclophilin D was expressed in E.coli and purified by conventional chromatography techniques.

Machida K., et al.(2006) J. Biol. Chem. 281(20):14314-20. Yokoi H., et al.(1996) Genomics ; 35(3):448-55.

4826932

NP_005029

NM_005038.2

42.9kDa (390aa), confirmed by MALDI-TOF.

C-MYB Transcription Factor Network Pathway 138073

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq, Jul 2008]

0.1 mg

375 USD