Human, His-tagged, Recombinant, E.coli | MyBioSource MBS203144 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Human, His-tagged, Recombinant, E.coli

catalog :

MBS203144

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203144

products type :

Recombinant Protein

products full name :

Human, His-tagged, Recombinant, E.coli

products short name :

14-3-3 eta

products name syn :

YWHA1; YWHAH; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide; 14-3-3 eta; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta polypeptide 14 3 3 protein eta; HGNC:12853; Protein AS1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide; YWHA 1.

other names :

14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 protein eta; 14-3-3 eta; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta; Protein AS1

products gene name :

14-3-3 eta

other gene names :

YWHAH; YWHAH; YWHA1; YWHA1

uniprot entry name :

1433F_HUMAN

sequence length :

246

sequence :

MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN

purity :

> 95% by SDS-PAGE

form :

Liquid in 20 mM Tris pH 8.0, 10% glycerol

concentration :

1 mg/ml ( determined by Absorbance at 280nm )

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag. Accession No.: NP_003396

other info2 :

Expression System: E Coli

products categories :

Signal Transduction

products description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, ?, ?, ?, ?, ?, ? and ? that have been identified in mammals. The 14-3-3 eta, a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3 eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3 eta, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

products references :

Sato S., et al. (2006) EMBO J 25(1):211-21. Chen J., et al. (2007), J Neurosci Res. 85(8):1724-33.

ncbi gi num :

4507951

ncbi acc num :

NP_003396

ncbi gb acc num :

NM_003405.3

ncbi mol weight :

30.3 kDa (266 aa), confirmed by MALDI-TOF

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Epstein-Barr Virus Infection Pathway (585562)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

uniprot summary :

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Protein type: Nuclear receptor co-regulator; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 22q12.3. Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: protein domain specific binding; insulin-like growth factor receptor binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding. Biological Process: intracellular protein transport; substantia nigra development; regulation of neuron differentiation; apoptosis; positive regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; glucocorticoid catabolic process; regulation of synaptic plasticity; negative regulation of dendrite morphogenesis; regulation of sodium ion transport

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage