Adenylate kinase 3(C22S), 1- 223aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203143 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Adenylate kinase 3(C22S), 1- 223aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203143

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203143

products type :

Recombinant Protein

products full name :

Adenylate kinase 3(C22S), 1- 223aa, Human, His-tag, Recombinant, E Coli

products short name :

Adenylate kinase 3

products name syn :

AK3; AKL3L; Adenylate kinase 3 alpha like 1; Adenylate kinase 3; AK3L1; AK6; AKL3L1; FIX; RP11 6J24.4.

other names :

adenylate kinase 4, mitochondrial; Adenylate kinase 4, mitochondrial; adenylate kinase 4, mitochondrial; AK 4; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase AK4, mitochondrial; adenylate kinase 3-like 1; adenylate kinase isoenzyme 4, mitochondrial; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase; adenylate kinase 4; Adenylate kinase 3-like; GTP:AMP phosphotransferase AK4

other gene names :

AK4; AK4; AK3; AK 4; AK3L1; AK3L2;

uniprot entry name :

KAD4_HUMAN

sequence length :

223

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY

purity :

> 90% by SDS - PAGE

form :

Liquid in 20mM Tris-HCl buffer (pH8.0), 2mMDTT, 20% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Signal Transduction

products description :

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Nobumoto M., et al.(1998) J. Biol. Chem. 123(1):128-35. Noma T., et al.(2001) J. Biol. Chem. 358(Pt 1):225-32.

ncbi gi num :

53832001

ncbi acc num :

NP_982289

ncbi gb acc num :

NM_203464.2

ncbi mol weight :

29.3 kDa (259 aa), confirmed by MALDI-TOF.

ncbi pathways :

Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (618580); Adenine Ribonucleotide Biosynthesis, IMP = ADP,ATP Pathway (468242); Metabolic Pathways (132956); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Adenosine Ribonucleotides De Novo Biosynthesis Pathway (835389); Purine Nucleotides De Novo Biosynthesis Pathway (142277); Superpathway Of Purine Nucleotide Salvage (907942)

ncbi summary :

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]

uniprot summary :

AK3L1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. May also be active with GTP. Belongs to the adenylate kinase family. Protein type: EC 2.7.4.10; Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.6; Mitochondrial. Chromosomal Location of Human Ortholog: 1p31.3. Cellular Component: mitochondrion; mitochondrial matrix. Molecular Function: GTP binding; nucleoside diphosphate kinase activity; adenylate kinase activity; nucleoside triphosphate adenylate kinase activity; ATP binding. Biological Process: ATP metabolic process; response to drug; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; brain development; AMP metabolic process; liver development; GTP metabolic process

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage