protein

Recombinant Liver X Receptor Alpha (LXRa)

MBS2031402

0.01 mg

150 USD

Recombinant Protein

Recombinant Liver X Receptor Alpha (LXRa)

[Liver X Receptor Alpha]

[liver X receptor alpha; Oxysterols receptor LXR-alpha; oxysterols receptor LXR-alpha; nuclear receptor subfamily 1, group H, member 3; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3]

[LXRa]

[NR1H3; NR1H3; LXRA; LXR-a; RLD-1; LXRA]

NR1H3_HUMAN

E Coli

447

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. NELCSV CGDKASGFHY NVLSCEGCKG FFRRSVIKGA RYVCHSGGHC PMDTYMRRKC QECRLRKCRQ AGMREECVLS EEQIRLKKMK RQEEEQAQAT SAPPRASSPP QVLPQLSPEQ LGMIEKLVAA QQLCNRRSFS DQLRVTPWPM APDPQSREAR QQRFAHFTEL AIVSVQEIVD FAKQLPGFLQ LSREDQIALL KTSAIEVMLL ETSRRYNPGS ESITFLKDFS YNREDFAKAG LQVEFINPIF EFSRAMNELQ LNDAEFALLI AISIFSADRP NVQDQLQVERLQHTYVEALH AYVSIHHPHD RLMFPRMLMK LVSLRTLSSV HSEQVFALRL QDKK

>99%

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Source: Prokaryotic expression. Residues: Asn95~Lys434 . Tags: N-terminal His-Tag . Tissue Specificity: Liver. Subcellular Location: Nucleus. Traits: Freeze-dried powder

Predicted isoelectric point: 8.6. Predicted Molecular Mass: 42.8kDa. Accurate Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions. Uage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

216409704

BAH02289.1

Adipogenesis Pathway (198832); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887)

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Nuclear receptor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex. Molecular Function: protein binding; ligand-dependent nuclear receptor activity; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity. Biological Process: negative regulation of lipid transport; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; positive regulation of cholesterol transport; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; sterol homeostasis; response to progesterone stimulus; negative regulation of macrophage activation; apoptotic cell clearance

0.01 mg

150 USD

0.05 mg

295

0.1 mg

460

0.2 mg

570

0.5 mg

1105

1 mg

1645