protein

Heat shock 60 kDa protein 1

MBS203138

0.1 mg

310 USD

Recombinant Protein

Heat shock 60 kDa protein 1

HSP60

CPN60; GroEL; HSP65; SPG13; HuCHA60; Heat shock 60kDa protein 1; HSP60; Heat shock 60kDa protein 1 60 kDa chaperonin; GroEL; E; coli; homolog of; 60 kDa heat shock protein mitochondrial; 60kDa; cb863; Chaperonin; Chaperonin 60; Chaperonin; 60-KD; CPN 60; fa04a05; fb22d10; fi27b05; GroEL Homolog; Heat shock 60kD protein 1 (chaperonin); Heat shock 60kD protein 1 chaperonin; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 1 (chaperonin); Heat Shock Protein 60; Heat shock protein 65; HLD4; Hsp 60; HSP 65; HSPD 1; HSPD1; HuCHA60; id:ibd2197; Spastic paraplegia 13 Mitochondrial matrix protein P1; P60 lymphocyte protein; sb:cb144; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (autosomal dominant); SPG 13; wu:fa04a05; wu:fb22d10; wu:fi04a12; wu:fi27b05.

60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; P60 lymphocyte protein; chaperonin 60; heat shock protein 65; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; heat shock 60kDa protein 1 (chaperonin); 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein

HSP60

HSPD1; HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60; HSP60; CPN60; HSP-60; Hsp60

CH60_HUMAN

573

MGSSHHHHHH SSGLVPRGSH MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF

> 95% by SDS - PAGE

Liquid in 20mM Tris-HCl buffer pH 8.0, containing 5mM DTT, 10% glycerol.

1mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Heat Shock Proteins

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding. Recombinant human HSP60, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Cheng MY., et al. (1990), Nature. 348: 455- 458. Ghosh JC., et al. (2008), J Biol Chem. Feb 22; 283(8):5188-94

31542947

NP_002147

NM_002156.4

63kDa (593aa)

Legionellosis Pathway (469200); Legionellosis Pathway (469186); Metabolism Of Proteins Pathway (106230); Mitochondrial Protein Import Pathway (576261); RNA Degradation Pathway (117291); RNA Degradation Pathway (116127); SIDS Susceptibility Pathways (198901); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743); Type I Diabetes Mellitus Pathway (83095)

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family. Protein type: Mitochondrial; Chaperone. Chromosomal Location of Human Ortholog: 2q33.1. Cellular Component: extracellular space; cell surface; protein complex; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex. Molecular Function: protein binding; p53 binding; lipopolysaccharide binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; double-stranded RNA binding; DNA replication origin binding; ATP binding; single-stranded DNA binding. Biological Process: caspase activation; B cell proliferation; T cell activation; viral reproduction; protein stabilization; B cell activation; positive regulation of apoptosis; positive regulation of interleukin-12 production; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; de novo protein folding; protein refolding; positive regulation of T cell mediated immune response to tumor cell; positive regulation of T cell activation; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; positive regulation of macrophage activation; negative regulation of apoptosis. Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4

0.1 mg

310 USD

0.5 mg

720