protein

TRAIL, 114-281aa, Human, Recombinant, E Coli

MBS203134

0.1 mg

255 USD

Recombinant Protein

TRAIL, 114-281aa, Human, Recombinant, E Coli

TRAIL

APO2L; Apo-2 ligand; Tumor necrosis factor ligand superfamily; member 10; TNFSF10; CD253; TL2; TRAIL; Tumor necrosis factor (ligand) superfamily; member 10; TNF-Related Apoptosis Inducing Ligand

tumor necrosis factor ligand superfamily member 10 isoform 1; Tumor necrosis factor ligand superfamily member 10; tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor (ligand) superfamily, member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253

TRAIL

TNFSF10; TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; APO2L; TRAIL; Apo-2L; Protein TRAIL

TNF10_HUMAN

281

MVRERGPQRV AAHITGTRGR SNTLSSPNSK NEKALGRKIN SWESSRSGHS FLSNLHLRNG ELVIHEKGFY YIYSQTYFRF QEEIKENTKN DKQMVQYIYK YTSYPDPILL MKSARNSCWS KDAEYGLYSI YQGGIFELKE NDRIFVSVTN EHLIDMDHEA SFFGAFLVG

> 95% by SDS - PAGE

Liquid in 20mM Tris pH 7.5, 300mM NaCl, 0.1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Cancer

TNF-related apoptosis-inducing ligand (TRAIL) belongs to the tumor necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumor cell lines upon binding to the death-signalling receptors on the cell membrane. TRAIL binds to one of four receptors that have been identified in humans, including TRAIL-R1/DR4, TRAIL-R2/KILLER/DR5, TRAIL-R3/DcR1/TRID, and TRAIL-R4/DcR2/TRuNDD. Both DR4 and DR5 are pro-apoptotic receptors, which contain a cytoplasmic death domain and mediate apoptosis on binding to TRAIL. By contrast, TRID and TRuNDD do not contain a cytoplasmic death domain and block the function of TRAIL by competing with DR4 and DR5 for binding of TRAIL. Recombinant human TRAIL was expressed in E.coli and purified by using conventional chromatography techniques.

Janssen EM., et al. (2005). Nature. 434(7029):88-93. Deocaris CC., et al. (2007) Ann N Y Acad Sci. 1119:165-75.

4507593

NP_003801

NM_003810.3

23 kDa (211 aa)

Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822!!Caspase-8 Activation By Cleavage Pathway 105654!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Death Receptor Signalling Pathway 105650!!Dimerization Of Procaspase-8 Pathway 105655

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

0.1 mg

255 USD