protein

Recombinant Sodium Iodide Symporter (NIS)

MBS2031325

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Recombinant Protein

Recombinant Sodium Iodide Symporter (NIS)

[Sodium Iodide Symporter]

[sodium iodide symporter; Sodium/iodide cotransporter; sodium/iodide cotransporter; solute carrier family 5 (sodium/iodide cotransporter), member 5; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5]

[NIS]

[SLC5A5; SLC5A5; NIS; TDH1; NIS; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter]

SC5A5_HUMAN

E. coli

> 90%

100mMNaHCO 3 , 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL, 5% Trehalose.

Original Concentration: 200µg/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-PAGE

Sequence

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Ser547~Leu643. Tags: N-terminal His and GST Tag. Subcellular Location: Membrane. Traits: Freeze-dried powder. Predicted isoelectric point: 4.9. Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Phenomenon Explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

1628579

AAB17378.1

Predicted Molecular Mass: 40.5kDa. Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.

Amine-derived Hormones Pathway (1270175); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158); Organic Anion Transporters Pathway (1269917); SLC-mediated Transmembrane Transport Pathway (1269907); Thyroid Hormone Synthesis Pathway (835410); Thyroid Hormone Synthesis Pathway (839541); Thyroxine (Thyroid Hormone) Production Pathway (920992); Thyroxine Biosynthesis Pathway (1270177); Transmembrane Transport Of Small Molecules Pathway (1269903)

This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]

SLC5A5: Mediates iodide uptake in the thyroid gland. Defects in SLC5A5 are the cause of thyroid dyshormonogenesis 1 (TDH1). A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: integral to membrane; plasma membrane; nucleus; vesicle. Molecular Function: iodide transmembrane transporter activity; sodium:iodide symporter activity. Biological Process: transport; iodide transport; thyroid hormone generation; ion transport; transmembrane transport. Disease: Thyroid Dyshormonogenesis 1

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