Recombinant Slit Homolog 2 (Slit2) | MyBioSource MBS2031306 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Slit Homolog 2 (Slit2)

catalog :

MBS2031306

quantity :

0.01 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2031306

products type :

Recombinant Protein

products full name :

Recombinant Slit Homolog 2 (Slit2)

products short name :

[Slit Homolog 2]

other names :

[slit homolog 2; Slit homolog 2; slit homolog 2 protein; Slit homolog 2]

products gene name :

[Slit2]

other gene names :

[SLIT2; SLIT2]

uniprot entry name :

K7CZV3_PANTR

host :

E.coli

reactivity :

Mouse

purity :

> 97%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300

concentration :

Original Concentration: 200ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residuess: Cys1408~Cys1519. Tags: N-terminal His Tag. Tissue Specificity: Secreted. Traits: Freeze-dried powder. Predicted isoelectric point: 8.8. Predicted Molecular Mass: 13.9kDa. Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

410304000

ncbi acc num :

Q9R1B9

ncbi pathways :

Axon Guidance Pathway (83781); Axon Guidance Pathway (476)

uniprot summary :

SLIT2: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth- stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post- crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Cellular Component: cytoplasm; extracellular space; plasma membrane; proteinaceous extracellular matrix. Molecular Function: chemorepellent activity; GTPase inhibitor activity; heparan sulfate proteoglycan binding; heparin binding; laminin-1 binding; protein binding; protein homodimerization activity; proteoglycan binding; receptor binding; Roundabout binding. Biological Process: axon extension involved in axon guidance; axon guidance; axonogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; cell-cell adhesion; central nervous system projection neuron axonogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; corticospinal neuron axon guidance through the spinal cord; dorsal/ventral axon guidance; in utero embryonic development; induction of negative chemotaxis; metanephros development; motor axon guidance; negative chemotaxis; negative regulation of actin filament polymerization; negative regulation of axon extension; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of inflammatory response; negative regulation of leukocyte chemotaxis; negative regulation of protein amino acid phosphorylation; negative regulation of small GTPase mediated signal transduction; negative regulation of smooth muscle cell migration; negative regulation of vascular permeability; neurite morphogenesis; olfactory bulb development; positive regulation of apoptosis; retinal ganglion cell axon guidance; telencephalon cell migration; ureteric bud development

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

280

size3 :

0.1 mg

price3 :

430

size4 :

0.2 mg

price4 :

530

size5 :

0.5 mg

price5 :

1020

size6 :

1 mg

price6 :

1520

more info or order :

MyBioSource product webpage