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Interleukin-3, 20-152aa Human, His-tag, Recombinant, E Coli

MBS203126

0.1 mg

375 USD

MBS203126

Recombinant Protein

Interleukin-3, 20-152aa Human, His-tag, Recombinant, E Coli

Interleukin-3

IL-3; Multipotential colony-stimulating factor(multi-CSF); Mast cell growth factor(MCGF); interleukin 3 (colony-stimulating factor; multiple); MuLTI-CSF; MCGF; multilineage-colony-stimulating factor; hematopoietic growth factor; P-cell stimulating factor; mast-cell growth factor

IL-3; Interleukin-3; interleukin-3; P-cell stimulating factor; P-cell-stimulating factor; colony-stimulating factor, multiple; hematopoietic growth factor; mast cell growth factor; mast-cell growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; interleukin 3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

IL3; IL3; IL-3; MCGF; MULTI-CSF; IL-3; MCGF

IL3_HUMAN

152

MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

> 90% by SDS - PAGE

Liquid in 20mM Tris pH 8.0, 0.2mM PMSF, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: The ED50 for this effect is Cell line: TF1 (human erythroleukemic cell) . Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF . Assay medium - 10% FBS RPMI 1640 without Gm-CSF . Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Starvation: 24hr, Assay medium . Incubation time: 24 hr (after sample treatment) . Concentration range: 0.048 ng/ml - 25 ng/ml . Detection method: MTT assay

Hormones & Cytokines

Interleukin-3(IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17 kDa (133 amino acids). It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19 amino acids. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84). Recombinant human IL-3 was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629. Michaud DS., et al. (2006), Cancer Res. 66(8):4525-30.

3002475

AAC08706

17.3kDa (154aa), confirmed by MALDI-TOF.

Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Asthma Pathway 83120!!Asthma Pathway 532!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Fc Epsilon RI Signaling Pathway 83082

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

0.1 mg

375 USD