catalog number :
MBS2031202
products type :
Recombinant Protein
products full name :
Recombinant NUAK Family SNF1 Like Kinase 1 (NUAK1)
products short name :
[NUAK Family SNF1 Like Kinase 1]
other names :
[NUAK family SNF1-like kinase 1; NUAK family SNF1-like kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; AMPK-related protein kinase 5; ARK5; Omphalocele kinase 1]
products gene name :
[NUAK1]
other gene names :
[NUAK1; NUAK1; ARK5; ARK5; KIAA0537; OMPHK1; ARK5]
uniprot entry name :
NUAK1_HUMAN
sequence :
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. KRSNSEH RSHSTGFIEG IVSPALPSPF KMEQDLCRTA IPLPSSPEAD MSGKLSLKQS ATMPKKGILK KTQQRESGYY SSPERSESSE LLDSNDVVIS GGLSSPPPDP ARGTSHSLSC RRKGILKHSS RYSDGGTDPA LTRPEMPTLE SLSPPGVPSD GISRSYSRPS SIISDDSVLS SDSFDLLELQ ENRPARQRIR SCVSAENFLQ LQDFETPHNR PRPQYLKRLA DSSFSLLTDMDDVTQVYKKA L
form :
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, WB, ELISA, IP, CoIP, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
Sequence Information
image2 heading :
SDS-Page
other info1 :
Source: Prokaryotic expression. Residues: Lys404~Leu651. Tags: N-terminal His-Tag. Subcellular Location: Nucleus. Cytoplasm. Traits: Freeze-dried powder
other info2 :
Predicted isoelectric point: 7.9. Predicted Molecular Mass: 57.2kDa. Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ncbi acc num :
NP_055655.1
ncbi gb acc num :
NM_014840.2
uniprot summary :
NuaK1: a protein kinase of the CAMKL family. Phosphorylated and activated by AKT1 during glucose starvation. May activate ATM kinase under conditions of nutrient starvation. Suppresses apoptosis induced by nutrient starvation and death receptors via inhibition of caspase 8 activation. Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMKL family; NuaK subfamily. Chromosomal Location of Human Ortholog: 12q23.3. Cellular Component: microtubule cytoskeleton; cytoplasm; nucleolus; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; metal ion binding; ATP binding. Biological Process: regulation of cell adhesion; cell adhesion; protein amino acid phosphorylation; response to DNA damage stimulus; regulation of cell proliferation