catalog number :
MBS203117
products type :
Recombinant Protein
products full name :
Mouse PRMT1 isoform2, 1-353aa, mouse, His-MBP tag, Recombinant, E Coli
products short name :
PRMT1 isoform2
products name syn :
Mrmt1; Hrmt112; AW214366; Protein arginine N-methytransferase 1; PRMT1 isoform2; PRMT1; Protein arginine N-methyltransferase 1 ANM 1; HCP 1; HMT1 hnRNP methyltransferase; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; HMT2; HRMT1 L2; HRMT1L 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; HMT 2; HMT1 (hnRNP methyltransferase S; cerevisiae) like 2; HMT1 hnRNP methyltransferase like 2; HMT1 hnRNP methyltransferase like 2 (S; cerevisiae); Human mRNA for suppressor for yeast mutant; Human mRNA for suppressor for yeast mutant complete cds; Interferon receptor 1 bound protein 4; Interferon receptor 1 bound protein4; Interferon receptor 1bound protein 4; IR1 B4; IR1B 4; Mrmt 1; Mrmt1; PRMT 1; R1B4; S; cerevisiae like 2; Protein arginine N methyltransferase 1; Protein arginine N methyltransferase1
other names :
protein arginine N-methyltransferase 1; Protein arginine N-methyltransferase 1; protein arginine N-methyltransferase 1; heterogeneous nuclear ribonucleoproteins methyltransferase-like 2; histone-arginine N-methyltransferase PRMT1; protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1 (EC:2.1.1.125)
products gene name :
PRMT1 isoform2
other gene names :
Prmt1; Prmt1; Mrmt1; Hrmt1l2; AW214366; 6720434D09Rik; Hrmt1l2; Mrmt1
uniprot entry name :
ANM1_MOUSE
sequence :
MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
purity :
> 90% by SDS - PAGE
form :
Liquid in 40mM Tris pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Mouse. Tag: His-MBP tag
other info2 :
Expression System: E Coli. Bioactivity: 10,000unit/mg. Assay: One unit will transfer 1pmol of methyl group to synthetic . peptide of histone H4 for 10 minutes at 37C.
products categories :
Histone Related
products description :
Protein arginine N-methyltransferase 1(PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1(AAF37293) shares 99.9% sequence identity with the human proteins(NP_938074). Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Herrmann F., et al. (2005). J Biol Chem. 280(45):38005-10. Abramovich C., et al. (1997). EMBO J. 16(2):260-6.
ncbi mol weight :
84 kDa (750 aa)
ncbi pathways :
Androgen Receptor Signaling Pathway 198319!!Chromatin Modifying Enzymes Pathway 1110662!!Chromatin Organization Pathway 1110661!!FoxO Signaling Pathway 921412!!RMTs Methylate Histone Arginines Pathway 1110667!!Tryptophan Metabolism Pathway 198332!!XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway 755427!!MRNA Processing Pathway 198369