protein

PAI-1, 24-402aa, Human, His-tag, Recombinant, E Coli

MBS203116

0.1 mg

310 USD

Recombinant Protein

PAI-1, 24-402aa, Human, His-tag, Recombinant, E Coli

PAI-1

PLANH1; SERPINE1; PAI1; PLANH1; Plasminogen activator inhibitor-1; Plasminogen activator inhibitor-1

plasminogen activator inhibitor 1; Plasminogen activator inhibitor 1; plasminogen activator inhibitor 1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Endothelial plasminogen activator inhibitor; Serpin E1

PAI-1

SERPINE1; SERPINE1; PAI; PAI1; PAI-1; PLANH1; PAI1; PLANH1; PAI; PAI-1

PAI1_HUMAN

402

MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP

> 95% by SDS - PAGE

In 150mM Na2HPO4, pH 6.6, 0.5mM NaCl, 2mM Glutathione, 0.01% Tween-80

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity, the IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC. Assay: 1. Prepare a recombinant PAI-1 with various concentrations (2,000 nM, 1,000 nM, 500 nM, 250 nM, 125 nM) in assay buffer. (Assay buffer: 50 mM Tris-HCl pH 8.0, 0.01% Tween 20). 2. Mix equal volumes of the PAI-1 protein and diluted 2 ug/ml uPA (Prospec. Cat No. ENZ-264). 3. Incubate at room temperature for 15 minutes. 4. After dilute incubated reaction mixtures (PAI-1, uPA) by a 5 fold dilution in assay buffer, mix 50ul of 200uM substrate (Z-GGR-AMC) and diluted mixtures. 4. Read excitation wavelength 355nm and emission wavelength 460nm for 10 minutes. - Z-GGR-AMC (CALBIOCHEM, Cat.No, 672159). - 96 well Polystyrene Microplate, black (greniner bio-one, Cat.No, 655077) . - Fluorescent plate reader (PerkinElmer, VICTORX3).

Enzymes & Proteases

Plasminogen activator inhibitor-1(PAI-1), a member of the serine protease inhibitor(serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Gorlatova NV., et al.(2007) J Biol Chem. 282(12):9288-96. Renckens R., et al. (2006) J Immunol. 177(11):8171-6.

10835159

NP_000593

NM_000602.4

45 kDa (400 aa)

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Blood Clotting Cascade Pathway (198840); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Circadian Clock Pathway (187173); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Direct P53 Effectors Pathway (137939)

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

SERPINE1: a secreted protein that acts as bait for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding. Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; transcription initiation from RNA polymerase II promoter; platelet activation; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression; blood coagulation; positive regulation of inflammatory response. Disease: Plasminogen Activator Inhibitor-1 Deficiency

0.1 mg

310 USD

0.5 mg

720