catalog number :
MBS2031156
products type :
Recombinant Protein
products full name :
Recombinant Calprotectin (CALPRO)
products short name :
[Calprotectin]
products gene name :
[CALPRO]
sequence :
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. LTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDINTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE
form :
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
tested application :
Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
Sequence Information
image2 heading :
SDS-Page
other info1 :
Source: Prokaryotic expression. Residues: Met1~Glu89 and Met1~Lys113. Tags: N-terminal His-Tag. Traits: Freeze-dried powder. Accurate Molecular Mass: 11&15kDa as determined by SDS-PAGE reducing conditions.
other info2 :
Phenomenon explanation: Calprotectin is a complex of two S100 calcium-binding proteins that are found primarily in granulocytes, some subsets of macrophages, and squamous epithelium; these proteins are also called the migration inhibitory-related proteins (MRP)-8 and MRP14, S100A8 and S100A9. Calprotectin is a 24kDa dimer of calcium binding proteins S100A8 and S100A9. After co-expression of S100A8 and S100A9, two bands of proteins can be observed, which means S100A8 and S100A9, respectively. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.