Recombinant Lamin B1 (LMNB1) | MyBioSource MBS2031124 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Lamin B1 (LMNB1)

catalog :

MBS2031124

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

image 3 :

SDS-PAGE

product information

catalog number :

MBS2031124

products type :

Recombinant Protein

products full name :

Recombinant Lamin B1 (LMNB1)

products short name :

[Lamin B1]

other names :

[Lamin B1; Lamin-B1; lamin-B1; lamin B1]

products gene name :

[LMNB1]

other gene names :

[LMNB1; LMNB1; LMN; ADLD; LMN2; LMNB; LMN2; LMNB]

uniprot entry name :

LMNB1_HUMAN

host :

E.coli

purity :

> 97%

form :

100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

concentration :

Original Concentration: 200ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).

image1 heading :

Sequence Information

image2 heading :

Gene Sequencing (Extract)

image3 heading :

SDS-PAGE

other info1 :

Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Ser52~Gly233. Tags: N-terminal His Tag. Subcellular Location: Nucleus

other info2 :

Traits: Freeze-dried powder. Predicted isoelectric point: 4.8. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

74354337

ncbi acc num :

AAI03724.1

ncbi mol weight :

Predicted Molecular Mass: 24.2kDa. Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.

ncbi pathways :

Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Breakdown Of The Nuclear Lamina Pathway (1270291); Caspase Cascade In Apoptosis Pathway (137974); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); DNA Damage/Telomere Stress Induced Senescence Pathway (1270429)

ncbi summary :

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]

uniprot summary :

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 5q23.2. Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope. Molecular Function: phospholipase binding; structural molecule activity. Biological Process: apoptosis; cell structure disassembly during apoptosis. Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

265

size3 :

0.1 mg

price3 :

410

size4 :

0.2 mg

price4 :

505

size5 :

0.5 mg

price5 :

980

size6 :

1 mg

price6 :

1445

more info or order :

MyBioSource product webpage