BHMT (Betaine-homocysteine methyltransferase) Human, His- tag, Recombinant E Coli | MyBioSource MBS203110 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

BHMT (Betaine-homocysteine methyltransferase) Human, His- tag, Recombinant E Coli

catalog :

MBS203110

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203110

products type :

Recombinant Protein

products full name :

BHMT (Betaine-homocysteine methyltransferase) Human, His- tag, Recombinant E Coli

products short name :

BHMT

products name syn :

BHMT; BHMT (Betaine-homocysteine methyltransferase); EC 2.1.1.5; Betaine--homocysteine S-methyltransferase 1; Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase; Betaine homocysteine S methyltransferase; Betaine homocysteine S methyltransferase 1; BHMT1

other names :

betaine--homocysteine S-methyltransferase 1; Betaine--homocysteine S-methyltransferase 1; betaine--homocysteine S-methyltransferase 1; epididymis secretory sperm binding protein Li 61p; betaine--homocysteine S-methyltransferase

products gene name :

BHMT

other gene names :

BHMT; BHMT; BHMT1; HEL-S-61p

uniprot entry name :

BHMT1_HUMAN

sequence length :

406

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ

purity :

> 95% by SDS - PAGE

form :

Liquid. 20mM Tris pH7.5, 20% glycerol.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Nandita, et al., Protein Expr Purif (2002) 25:73-80. Breksa, A P et al., Biochemistry (1999) 38:13991-8.

ncbi gi num :

157266337

ncbi acc num :

NP_001704

ncbi gb acc num :

NM_001713.2

ncbi mol weight :

49.2 kDa (443 aa), confirmed by MALDI-TOF.

ncbi pathways :

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Glycine, Serine And Threonine Metabolism Pathway (82949); Glycine, Serine And Threonine Metabolism Pathway (313); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); One Carbon Metabolism Pathway (198756); Sulfur Amino Acid Metabolism Pathway (530753); Trans-sulfuration And One Carbon Metabolism Pathway (920958)

ncbi summary :

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

uniprot summary :

BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. Protein type: Methyltransferase; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.5. Chromosomal Location of Human Ortholog: 5q14.1. Cellular Component: protein complex; cytoplasm; cytosol. Molecular Function: betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding. Biological Process: S-methylmethionine cycle; betaine catabolic process; protein amino acid methylation; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage