protein

IRF1, 1-114aa Human, His-tag, Recombinant, E Coli

MBS203098

0.1 mg

310 USD

Recombinant Protein

IRF1, 1-114aa Human, His-tag, Recombinant, E Coli

IRF1

MAR; IRF1; Interferon regulatory factor 1; Interferon regulatory factor-1 Interferon regulatory factor 1; IRF 1; IRF-1; MAR1

interferon regulatory factor 1; Interferon regulatory factor 1; interferon regulatory factor 1; interferon regulatory factor 1 isoform +I9; interferon regulatory factor 1 isoform d78; interferon regulatory factor 1 isoform delta4; interferon regulatory factor 1 isoform delta7; interferon regulatory factor 1

IRF1

IRF1; IRF1; MAR; IRF-1; IRF-1

IRF1_HUMAN

325

MGSSHHHHHH SSGLVPRGSH MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPP

> 90% by SDS - PAGE

In 20mM Tris 8.0, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Hormones & Cytokines

IRF1, Interferon regulatory factor 1, is a member of the interferon regulatory transcription factor (IRF) family which regulates gene expression critical to immune response, hematopoiesis and proliferation. IRF-1 is a transcriptional activator for IFN-alpha, IFN-beta, and IFN-gamma stimulated genes. IRF1 is also a tumor suppressor transcription factor inducing apoptosis of tumorigenic cell lines. Recombinant human IRF1 was expressed in E.coli and purified by using conventional chromatography techniques.

Hochhaus A., et al. (1997) Leukemia. 11(7):933-9. Liu J., et al. (2005) J Biol Chem. 280(26):24347-55.

4504721

NP_002189

NM_002198.2

15 kDa (134 aa), confirmed by MALDI-TOF.

Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hemostasis Pathway (106028); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); IFN-gamma Pathway (138040); IL-7 Signaling Pathway (198857); IL12 Signaling Mediated By STAT4 Pathway (137936)

IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. [provided by RefSeq, Jul 2008]

IRF1: a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon- stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58, TRAIL, OAS1/2, PIAS1, PKR and RSAD2; antibacterial response, such as iNOS; anti- proliferative response, such as p53, LOX and CDKN1A; apoptosis, such as PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, COX-2 and CYBB; DNA damage responses and DNA repair, such as POLQ; MHC class I expression, such as TAP1, PSMB9, PSME1, PSME2 and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells. Defects in IRF1 are a cause of gastric adenocarcinoma (GASC), accounting for most of all gastric malignant tumors. Deletions or rearrangements of IRF1 can occur in preleukemic myelodysplastic syndrome (MDS) and acute myelogenous leukemia (AML). Protein type: Transcription factor; Tumor suppressor. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; nucleus; cytosol. Molecular Function: protein binding; DNA binding. Biological Process: transcription from RNA polymerase II promoter; CD8-positive, alpha-beta T cell differentiation; apoptosis; regulation of cell cycle; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of innate immune response; negative regulation of cell proliferation; positive regulation of interleukin-12 biosynthetic process; regulation of adaptive immune response; negative regulation of regulatory T cell differentiation; positive regulation of interferon type I production; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; cell cycle arrest; negative regulation of transcription, DNA-dependent; blood coagulation; defense response to virus. Disease: Gastric Cancer; Lung Cancer

0.1 mg

310 USD

0.5 mg

720