Acidic Fibroblast Growth Factor(aFGF), Human, Recombinant, E Coli | MyBioSource MBS203092 product information

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product summary

company name :

MyBioSource

product type :

protein

product name :

Acidic Fibroblast Growth Factor(aFGF), Human, Recombinant, E Coli

catalog :

MBS203092

quantity :

0.1 mg

price :

310 USD

product information

catalog number :

MBS203092

products type :

Recombinant Protein

products full name :

Acidic Fibroblast Growth Factor(aFGF), Human, Recombinant, E Coli

products short name :

Acidic Fibroblast Growth Factor

products name syn :

FGF1; FGFA; Acidic Fibroblast Growth Factor (aFGF); HBGF-1; Acidic fibroblast growth factor; aFGF; Beta-endothelial cell growth factor; ECGF-beta; Heparin-binding growth factor 1; Acidic Fibroblast Growth Factor Acidic fibroblast growth factor; AFGF; Beta endothelial cell growth factor; ECGF; ECGF beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fibroblast growth factor 1; Fibroblast Growth Factor 1 Acidic; GLIO703; HBGF 1; HBGF1; Heparin binding growth factor 1; Heparin binding growth factor 1 precursor.

other names :

fibroblast growth factor 1 isoform 1; Fibroblast growth factor 1; fibroblast growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic); Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1

products gene name :

aFGF

other gene names :

FGF1; FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha; FGFA; FGF-1; aFGF; ECGF; HBGF-1

uniprot entry name :

FGF1_HUMAN

sequence length :

155

sequence :

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

purity :

> 95% by SDS - PAGE

form :

Liquid. 20 mM Tris, pH 7.0, 100 mM NaCl

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: The ED50 for this effect is 340,000 units/ml). Assay: 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) . 2. Maintenance Condition: RPMI 1640 containing 10% FBS . 3. Assay Medium: serum free RPMI 1640 . 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) . 5. Serum Free Starvation: 24hr with RPMI1640 . 6. Incubation Time: 24 hr (after sample treatment) . 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution) . 8. Detection method: Brdu assay

products categories :

Hormones & Cytokines

products description :

Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.

products references :

Jaye M. et al., (1986) Science. 233, 541-544. Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129. Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. u.S.A. 97, 49-54.

ncbi gi num :

4503697

ncbi acc num :

NP_000791

ncbi gb acc num :

NM_000800.4

ncbi mol weight :

15.9kDa (141aa), confirmed by MALDI-TOF.

ncbi pathways :

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

uniprot summary :

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 5q31. Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; nucleolus; extracellular region; cell cortex; cytosol. Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of cell division; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720