Recombinant Nicastrin (NCSTN) | MyBioSource MBS2030917 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Nicastrin (NCSTN)

catalog :

MBS2030917

quantity :

0.01 mg

price :

145 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2030917

products type :

Recombinant Protein

products full name :

Recombinant Nicastrin (NCSTN)

products short name :

[Nicastrin]

other names :

[nicastrin isoform 1; Nicastrin; nicastrin; nicastrin]

products gene name :

[NCSTN]

other gene names :

[NCSTN; NCSTN; ATAG1874; KIAA0253]

uniprot entry name :

NICA_HUMAN

host :

E.coli

purity :

>95%

form :

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

concentration :

200 ug/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition

tested application :

Positive Control; Immunogen; SDS'PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

SDS-Page

other info1 :

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression . Residues: Val35~Gln534. Tags: N'terminal His Tag. Subcellular Location: Membrane. Traits: Freeze'dried powder. Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

other info2 :

Predicted isoelectric point: 5.2. Predicted Molecular Mass: 59.1KDa. Accurate Molecular Mass: 59kDa as determined by SDS'PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

24638433

ncbi acc num :

NP_056146.1

ncbi gb acc num :

NM_015331.2

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (1269536); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Axon Guidance Pathway (1270303); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (1269447); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (1268891); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (1268889); Degradation Of The Extracellular Matrix Pathway (1270257); Delta-Notch Signaling Pathway (198879)

ncbi summary :

This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]

uniprot summary :

nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q22-q23. Cellular Component: Golgi apparatus; focal adhesion; membrane; lysosomal membrane; integral to plasma membrane; endoplasmic reticulum; melanosome; plasma membrane; integral to membrane. Molecular Function: protein binding; endopeptidase activity. Biological Process: positive regulation of catalytic activity; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; myeloid cell homeostasis; membrane protein ectodomain proteolysis; Notch receptor processing; proteolysis; T cell proliferation; extracellular matrix disassembly; epithelial cell proliferation; amyloid precursor protein catabolic process; beta-amyloid metabolic process; ephrin receptor signaling pathway; protein processing. Disease: Acne Inversa, Familial, 1

size1 :

0.01 mg

price1 :

145 USD

size2 :

0.05 mg

price2 :

275

size3 :

0.1 mg

price3 :

430

size4 :

0.2 mg

price4 :

525

size5 :

0.5 mg

price5 :

1015

size6 :

1 mg

price6 :

1515

more info or order :

MyBioSource product webpage