Interleukin-18 Human, Recombinant, E Coli | MyBioSource MBS203090 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Interleukin-18 Human, Recombinant, E Coli

catalog :

MBS203090

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203090

products type :

Recombinant Protein

products full name :

Interleukin-18 Human, Recombinant, E Coli

products short name :

Interleukin-18

products name syn :

IL18; IGIF; IL1F4; Interleukin-18; IL-18; Interferon-gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Iboctadekin; Interleukin-18; IFN gamma inducing factor; IL 1 gamma; IL 18; IL 1g; IL1 gamma; IL18 protein; IL1g; IL1gamma; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin 18; Interleukin18

other names :

interleukin-18 isoform 1 proprotein; Interleukin-18; interleukin-18; IFN-gamma-inducing factor; IL-1 gamma; iboctadekin; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; interleukin 18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma

other gene names :

IL18; IL18; IGIF; IL-18; IL-1g; IL1F4; IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma

uniprot entry name :

IL18_HUMAN

sequence length :

193

sequence :

MYFGKLESKL SVIRNLNDQV LFIDQGNRPL FEDMTDSDCR DNAPRTIFII SMYKDSQPRG MAVTISVKCE KISTLSCENK IISFKEMNPP DNIKDTKSDI IFFQRSVPGH DNKMQFESSS YEGYFLACEK ERDLFKLILK KEDELGDRSI MFTVQNED

purity :

> 95% by SDS - PAGE

form :

Liquid. In Phosphate buffer saline (pH 7.4)

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Measured by its ability to induce IFN-gamma secretion using KG-1 human myeloid leukemia cells in the presence of TNF-alpha (20 ng/ml). The ED50 for this effect is < 0.85 ug/ml. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Cell line: KG-1 (human myeloid leukemia cell). Maintenance condition: 20% FBS RPMI 1640 . Assay medium: 1% FBS RPMI 1640 with TNF-alpha 20 ng/ml. Cell density: 3 x 104 cells/well (96 well plate). Incubation time: 24hr (after sample treatment)Concentration range: 300 pg/ml - 10 ug/ml. Detection method: IFN-gamma ELISA Kit (BD biosciences, Cat. No. 555142).

products categories :

Hormones & Cytokines

products description :

Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

products references :

Okamura H., et al. (1995). Nature, 378, 88-91. Yoshimoto TK., et al. (1998) J. Immunol, 161, 3400-3407.

ncbi gi num :

4504653

ncbi acc num :

NP_001553

ncbi gb acc num :

NM_001562.3

ncbi mol weight :

18.3kDa (158aa), confirmed by MALDI-TOF.

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cellular Roles Of Anthrax Toxin Pathway (138076); Corticotropin-releasing Hormone Pathway (920957); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Hypertrophy Model Pathway (198828)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

uniprot summary :

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 11q22.2-q22.3. Cellular Component: extracellular space; extracellular region; cytosol. Molecular Function: protein binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; regulation of cell adhesion; positive regulation of interleukin-17 production; T-helper 1 type immune response; positive regulation of natural killer cell proliferation; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage