product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Interleukin-2 (C145S) Human, Recombinant, E Coli
catalog :
MBS203089
quantity :
0.1 mg
price :
375 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203089
products type :
Recombinant Protein
products full name :
Interleukin-2 (C145S) Human, Recombinant, E Coli
products short name :
Interleukin-2
products name syn :
IL-2; TCGF; Aldesleukin; T-cell growth factor; Interleukin-2; IL2; interleukin 2
other names :
interleukin-2; Interleukin-2; interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion; interleukin 2; T-cell growth factor; TCGF; INN: Aldesleukin
other gene names :
IL2; IL2; IL-2; TCGF; lymphokine; IL-2; TCGF
uniprot entry name :
IL2_HUMAN
sequence length :
153
sequence :
MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
purity :
> 95% by SDS - PAGE
form :
Liquid. In Phosphate buffer saline (pH 7.4)
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human
other info2 :
Expression System: E Coli. Bioactivity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is Cell line: CTLL2 (mouse cytotoxic Tcell) . Maintenance Condition: 10% FBS RPMI 1640 with hIL2 . Assay medium: 10% FBS RPMI 1640 without hIL2 . Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Starvation: 24hr, Assay medium . Incubation Time: 24hr (after sample treatment) . Concentration Range: 0.039 ng/ml - 40 ng/ml . Detection method: MTT assay
products categories :
Hormones & Cytokines
products description :
IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
products references :
Morgan DA., et al. (1976). Science, 193. 1007-8. Watson J., et al. (1980). Immunol Rev, 51. 257-78. Taniguchi T., et al. (1983). Nature, 302. 305-10.
ncbi gi num :
28178861
ncbi acc num :
NP_000577
ncbi gb acc num :
NM_000586.3
ncbi mol weight :
15.4kDa (133aa), confirmed by MALDI-TOF.
ncbi pathways :
AhR Pathway 755436!!Allograft Rejection Pathway 83123!!Allograft Rejection Pathway 535!!Autoimmune Thyroid Disease Pathway 83121!!Autoimmune Thyroid Disease Pathway 533!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Corticotropin-releasing Hormone Pathway 920957
ncbi summary :
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
size :
0.1 mg
price :
375 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
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