protein

IGF-1, Human, Recombinant, E Coli

MBS203085

0.1 mg

255 USD

Recombinant Protein

IGF-1, Human, Recombinant, E Coli

IGF-1

Insulin-like growth factor-1; IGF-IA; Somatomedin-C; MGF; IBP1; IGF1; IBP1; IGF-1; Insulin-like growth factor-1 IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1; Insulin like growth factor 1 (somatomedin C); Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; Somatomedin C

insulin-like growth factor I isoform 4 preproprotein; Insulin-like growth factor I; insulin-like growth factor I; IGF-IA; IGF-IB; MGF; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C; insulin-like growth factor 1 (somatomedin C); Mechano growth factor; MGF; Somatomedin-C

IGF-1

IGF1; IGF1; IGFI; IGF-I; IGF1A; IBP1; IGF-I; MGF

IGF1_HUMAN

153

MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A

> 95% by SDS - PAGE

Liquid. In phosphate-buffered saline (PBS), pH 7.4

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: The ED50 for this effect is < 3.6 ng/ml. Measured in a cell proliferation assay using MCF7 cell. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Cell line: MCF7 (human) . Maintenance Condition: RPMI 1640 containing 10% FBS . Assay Medium: serum free RPMI 1640 . Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Serum Free Starvation: 24hr with RPMI1640 . Incubation Time: 40 hr (after sample treatment) . Concentration Range: 0.39ng/ml - 25ng/ml . Detection Method: Brdu assay

Hormones & Cytokines

The Insulin-like growth factor-1(IGF-1) is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Rinderknecht E., et al (1978) J Biol.Chem, 253: 2769. Zumstein P., et al (1987) J Biol. Chem, 262: 11252. Rabinovsky ED (2004) Neurol Res, 26: 204-210.

11024682

NP_000609

NM_000618.3

7.7kDa (71aa), confirmed by MALDI-TOF.

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198812)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region; plasma membrane. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; insulin receptor binding. Biological Process: muscle development; positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; glycolate metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; platelet activation; positive regulation of protein import into nucleus, translocation; positive regulation of mitosis; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; regulation of gene expression; response to heat; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; positive regulation of smooth muscle cell migration; signal transduction; positive regulation of activated T cell proliferation; negative regulation of cell proliferation; platelet degranulation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; DNA replication; skeletal development; positive regulation of granule cell precursor proliferation; phosphoinositide-mediated signaling; multicellular organism growth; regulation of multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; cellular protein metabolic process; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; Ras protein signal transduction; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell motility; blood coagulation; positive regulation of DNA replication. Disease: Insulin-like Growth Factor I Deficiency

0.1 mg

255 USD

0.5 mg

535