protein

EGF, Human, Recombinant, E Coli

MBS203083

0.1 mg

285 USD

Recombinant Protein

EGF, Human, Recombinant, E Coli

EGF

Epidermal growth factor; Pro-epidermal growth factor; urogastrone; Epidermal growth factor; EGF; epidermal growth factor (beta-urogastrone)

pro-epidermal growth factor isoform 1 preproprotein; Pro-epidermal growth factor; pro-epidermal growth factor; beta-urogastrone; epidermal growth factor; Urogastrone

EGF

EGF; EGF; URG; HOMG4; EGF

EGF_HUMAN

1207

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

> 95% by SDS - PAGE

Liquid. In phosphate-buffered saline(PBS), pH 7.4

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: The ED50 for this effect is < 0.89 ng/ml. Measured in a cell proliferation assay using mouse Balb3T3 cell. Assay: Cell line: Balb-3T3 (Mus musculus (mouse)) . Maintenance Condition: RPMI 1640 containing 10% FBS . Assay medium: serum free RPMI 1640 . Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Serum Free Starvation: 24hr with RPMI1640 . Incubation time: 24 hr (after sample treatment) . Concentration range: 1 pg/ml - 1 ug/ml . Detection method: Brdu assay

Hormones & Cytokines

Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Riese., et al (1998) Bioessays. 20: 41-48. Cohen S., (1983) Cancer. 15: 1787-1791. Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.

166362728

NP_001954

NM_001963.4

6.3kDa (54aa), confirmed by MALDI-TOF.

Adaptive Immune System Pathway (366160); Arf6 Signaling Events Pathway (138034); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Ceramide Signaling Pathway (138023); Choline Metabolism In Cancer Pathway (1059539); Choline Metabolism In Cancer Pathway (1084232); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Constitutive Signaling By EGFRvIII Pathway (1127578); Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway (530769)

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Hormone; Ligand, receptor tyrosine kinase. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: extracellular space; lysosomal membrane; plasma membrane; extracellular region; integral to membrane. Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; platelet activation; peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; transmembrane receptor protein tyrosine kinase activation (dimerization); phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of mitosis; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; positive regulation of epidermal growth factor receptor activity; branching morphogenesis of a tube; positive regulation of cell proliferation; innate immune response; negative regulation of secretion; angiogenesis; DNA replication; blood coagulation; positive regulation of phosphorylation; positive regulation of granule cell precursor proliferation; STAT protein nuclear translocation; positive regulation of DNA binding. Disease: Hypomagnesemia 4, Renal

0.1 mg

285 USD

0.5 mg

640