protein

GM-CSF, Human, Recombinant, E Coli

MBS203082

0.1 mg

310 USD

Recombinant Protein

GM-CSF, Human, Recombinant, E Coli

GM-CSF

CSF2; GMCSF; GM-CSF; GM-CSF; Colony-stimulating factor; CSF; Sargramostim; Molgramostin; Granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony stimulating factor Burst Promoting Activity; CMCSF; Colony stimulating factor; Colony Stimulating Factor 2; CSF 2; CSF Alpha; Eosinophil Colony Stimulating Factor; Granulocyte Macrophage Colony Stimulating Factor; MGC131935; MGC138897; Pluripoietin Alpha

granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; granulocyte macrophage-colony stimulating factor; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; Molgramostin; Sargramostim

GM-CSF

CSF2; CSF2; GMCSF; GMCSF; GM-CSF; CSF

CSF2_HUMAN

144

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

> 95% by SDS - PAGE

Liquid. In phosphate-buffered saline (PBS), pH 7.4

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: Cell line: TF1 (human erythroleukemic cell) . Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF . Assay medium: 10% FBS RPMI 1640 without Gm-CSF . Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . Starvation: 24hr, Assay medium . Incubation time: 24 hr (after sample treatment) . Concentration range: 0.048 ng/ml - 25 ng/ml . Detection method: MTT assay

Hormones & Cytokines

Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80. Wong GG, et al (1985) Science, 228, 810-815.

27437030

NP_000749

NM_000758.3

14.6 kDa (128 aa), confirmed by MALDI-TOF.

Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!Fc Epsilon RI Signaling Pathway 83082!!Fc Epsilon RI Signaling Pathway 493

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

0.1 mg

310 USD