protein

Recombinant Dickkopf Related Protein 3 (DKK3)

MBS2030790

0.01 mg

140 USD

Recombinant Protein

Recombinant Dickkopf Related Protein 3 (DKK3)

[Dickkopf Related Protein 3]

[dickkopf-related protein 3; Dickkopf-related protein 3; dickkopf-related protein 3; dickkopf WNT signaling pathway inhibitor 3]

[DKK3]

[DKK3; DKK3; RIG; REIC; REIC; Dickkopf-3; Dkk-3; hDkk-3]

DKK3_HUMAN

E Coli

>95%

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose

Original Concentration: 900 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression. Residues: Pro41~Ile348. Tags: N-terminal His Tag. Subcellular Location: Secreted. Traits: Freeze-dried powder

Predicted isoelectric point: 4.5. Predicted Molecular Mass: 38.2KDa. Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:. 1.Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. USAGE: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

66346689

NP_001018067.1

NM_001018057.1

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular space. Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent

0.01 mg

140 USD

0.05 mg

265

0.1 mg

410

0.2 mg

505

0.5 mg

980

1 mg

1445